Destruction and solubilization of supported phospholipid bilayers on silica by the biosurfactant surfactin

Langmuir : the ACS Journal of Surfaces and Colloids
Hsin-Hui ShenG Fragneto

Abstract

The lipopeptide surfactin from Bacillus subtilis strains exhibits strong surface and biological activity, the latter probably because of its interaction with biological membranes. We have investigated the interaction of aqueous solutions of surfactin with supported bilayers of diphosphatidylcholine (DPPC) on silica using neutron reflectometry. We have also used small-angle neutron scattering (SANS) to study the solubilized aggregates formed as a result of the destruction of the supported membrane by surfactin. Although surfactin on its own does not attach to the silica supporting surface, it is taken up from solution by the membrane, confirming that there is an attractive interaction between DPPC and surfactin. The surfactin concentration in the layer can reach up to about 20 mol % relative to DPPC. The membrane is stable provided that the surfactin concentration is below its critical micelle concentration (cmc, 5 x 10(-5) M). Above the cmc, however, the membrane is solubilized and removed from the surface, though not always completely, over a period of hours. There are signs that there is an induction period while the surfactin concentration builds up in the membrane. This would be consistent with the need for a threshold conc...Continue Reading

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Nov 12, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Hsin-Hui ShenPhil Taylor

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Citations

Dec 8, 2011·Analytical Chemistry·Irep Gözen, Aldo Jesorka
Oct 25, 2014·Nature Communications·Hsin-Hui ShenTrevor Lithgow
Jun 1, 2011·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Christophe DéjugnatThomas Zemb
Jul 13, 2013·Biochimica Et Biophysica Acta·Gabriela SeydlováMiroslav Pátek
Apr 22, 2014·Biochimica Et Biophysica Acta·Kamil WojciechowskiThomas Geue
Nov 20, 2012·Biochimica Et Biophysica Acta·Magali DeleuMarie-Paule Mingeot-Leclercq
Mar 31, 2011·The Journal of Physical Chemistry. B·Hsin-Hui ShenJeffrey Penfold
Dec 14, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yue DingHsin-Hui Shen
Feb 28, 2019·Proceedings of the National Academy of Sciences of the United States of America·Jhih-Hang JiangAnton Y Peleg
Oct 28, 2019·Advances in Colloid and Interface Science·J Penfold, R K Thomas
Sep 11, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Olga S OstroumovaLudmila V Schagina

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