PMID: 9535119Apr 16, 1998Paper

Destruction of locus coeruleus neuronal perikarya after injection of anti-dopamine-B-hydroxylase immunotoxin into the olfactory bulb of the rat

Neuroscience Letters
W W BlessingR G Wiley

Abstract

Saporin, a ribosome-inactivating protein, was coupled to a monoclonal antibody to dopamine-B-hydroxylase (DBH) and injected unilaterally into the olfactory bulb of rats. After 4-13 days survival, the rat brain was processed histologically and the locus coerulei (LC) examined with Nissl and anti-DBH staining. There were degenerating dendrites in surviving LC neurons on the side ipsilateral to the immunotoxin-injected olfactory bulb. The number of Nissl-positive LC neurons in a transverse section through the caudal one third of the LC was reduced from 116+/-10 to 50+/-8 neurons (P < 0.01, n = 7) and the number of DBH-positive neurons in the more rostral LC sections was reduced from 13+/-2 to 5+/-1 (P < 0.05, n = 4). Our results indicate that it is possible to lesion LC neurons via retrograde intraaxonal transport of saporin-anti-DBH immunotoxin from the olfactory bulb.

References

Mar 12, 1976·Brain Research·M G ZieglerD M Jacobowitz
Feb 15, 1991·The Journal of Comparative Neurology·J H McLean, M T Shipley

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Citations

Nov 14, 2000·Journal of Neuroscience Methods·R G Wiley, R H Kline IV
Nov 14, 2000·Journal of Neuroscience Methods·I J Llewellyn-SmithJ B Minson
Aug 26, 2003·Advanced Drug Delivery Reviews·Ronald G Wiley, Douglas A Lappi
Apr 15, 1999·Neuroreport·I J Llewellyn-SmithJ B Minson
May 19, 2004·The European Journal of Neuroscience·Carlos Blanco-CenturionPriyattam J Shiromani
Aug 5, 2006·Physiology & Behavior·Sue RitterAi-Jun Li
Jun 13, 2015·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Ai-Jun LiSue Ritter
Apr 22, 2016·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Mazher MohammedWilliam W Blessing
Aug 12, 2000·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·A M Schreihofer, P G Guyenet

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