Destruction of rat pancreatic islet beta-cells by cytokines involves the production of cytotoxic aldehydes

Endocrinology
W L Suarez-PinzonA Rabinovitch

Abstract

Cytokines produced by mononuclear leukocytes infiltrating pancreatic islets are candidate mediators of islet beta-cell destruction in autoimmune insulin-dependent diabetes mellitus. Cytokines may damage islet beta-cells by inducing oxygen free radical production in the beta-cells. Lipid peroxidation and aldehyde production are measures of oxygen free radical-mediated cell injury. In the current study, we used a HPLC technique to measure levels of different aldehydes produced in rat islets incubated with cytokines. The cytokine combination of interleukin-1beta (10 U/ml), tumor necrosis factor-alpha (10(3) U/ml), and interferon-gamma (10(3) U/ml), and the oxidant, t-butylhydroperoxide, induced significant increases in islet levels of the same aldehydes: butanal, pentanal, 4-hydroxynonenal (4-HNE), and hexanal. Cytokine-induced aldehyde production was associated with islet beta-cell destruction. Thus, cytokine-induced increases in malondialdehyde (MDA; at 4 h) and 4-HNE (at 8 h) preceded islet cell destruction (at 16 h), and the addition of 4-HNE, hexanal, MDA, and pentanal (1-200 microM) to th islets, but not other aldehydes at similar concentrations, produced dose-dependent destruction of islet beta-cells. Furthermore, an antiox...Continue Reading

Citations

Jan 26, 2010·The Journal of Surgical Research·Christoph LichtensternMarkus A Weigand
Jan 6, 2009·Annals of the New York Academy of Sciences·Jing ChenClayton E Mathews
Nov 8, 2011·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·M McCallA M J Shapiro
Mar 26, 2016·Molecular Aspects of Medicine·Giuseppe MaulucciShlomo Sasson
Aug 27, 2013·Free Radical Biology & Medicine·Guy CohenShlomo Sasson
Aug 31, 2002·Diabetes/metabolism Research and Reviews·P J DickinsonA J M Boulton
Apr 3, 2008·Cell Biochemistry and Function·Maurício da Silva Krause, Paulo Ivo Homem de Bittencourt
Jul 24, 2010·Pflügers Archiv : European journal of physiology·Gisela DrewsMartina Düfer
Apr 13, 2018·The Journal of Endocrinology·Ruben RodriguezRudy M Ortiz
Sep 15, 2010·Free Radical Research·Anne Negre-SalvayreNeven Zarkovic
Aug 22, 2017·Frontiers in Endocrinology·Undurti N Das
Apr 30, 2003·Transplantation·Thomas T TitusDerek W R Gray
Feb 10, 1999·The American Journal of Physiology·H R OliveiraA R Carpinelli
Apr 28, 2016·American Journal of Physiology. Endocrinology and Metabolism·Keiko OmoriYoko Mullen
Apr 5, 2000·The Journal of Biological Chemistry·C BonnyG Waeber
Aug 27, 1998·Biochemical Pharmacology·A Rabinovitch, W L Suarez-Pinzon

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