Desulfovibrio sp. genes involved in the respiration of sulfate during metabolism of hydrogen and lactate.

Applied and Environmental Microbiology
Jennifer L StegerLee R Krumholz

Abstract

To develop a better understanding of respiration by sulfate-reducing bacteria, we examined transcriptional control of respiratory genes during growth with lactate or hydrogen as an electron donor. RNA extracts of Desulfovibrio desulfuricans subsp. aestuarii were analyzed by using random arbitrarily primed PCR. RNA was reverse transcribed under low-stringency conditions with a set of random primers, and candidate cDNAs were cloned, sequenced, and characterized by BLAST analysis. Putative differentially expressed transcripts were confirmed by Northern blot analysis. Interestingly, dissimilatory bisulfite reductase was upregulated in the presence of hydrogen. To link these transcriptional changes to the physiology of sulfate-reducing bacteria, sulfide was measured during growth of several strains of Desulfovibrio on hydrogen or lactate, and this revealed that hydrogen-grown cells produced more sulfide per unit of cell mass than lactate-grown cells. Transcription of other redox proteins was characterized by Northern blotting to determine whether or not they were also transcribed to higher levels in hydrogen-grown cells. Growth on lactate produced greater transcription of [NiFe] hydrogenase. H(2)-grown cells transcribed the adenylyl...Continue Reading

References

Jun 1, 1979·Microbiological Reviews·W E BalchR S Wolfe
Oct 1, 1994·Protein Science : a Publication of the Protein Society·C R Goward, D J Nicholls
Mar 1, 1993·Nature Genetics·W Gish, D J States

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Citations

Jul 5, 2003·Journal of Microbiological Methods·In Seop ChangLee R Krumholz
Apr 23, 2010·The ISME Journal·Kim M HandleyJonathan R Lloyd
Sep 9, 2011·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Takeshi AkaoHitoshi Shimoi
Jan 9, 2004·Applied and Environmental Microbiology·Dwayne A EliasLee R Krumholz
Mar 10, 2004·Applied and Environmental Microbiology·In Seop ChangLee R Krumholz
Nov 5, 2005·Applied and Environmental Microbiology·John M SenkoLee R Krumholz
Feb 27, 2014·Environmental Science & Technology·Maria AlexandrinoMaria C Costa
Oct 18, 2005·Journal of Molecular Biology·Laetitia PieulleE Claude Hatchikian
Aug 9, 2003·Biochimica Et Biophysica Acta·Ricardo H PiresInês A C Pereira
Feb 24, 2007·Biochimica Et Biophysica Acta·Catarina M PaqueteAntónio V Xavier
Aug 21, 2014·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Catarina I P NunesMarta S P Carepo
Jun 14, 2005·Progress in Biophysics and Molecular Biology·Pedro M MatiasMaria Arménia Carrondo
Feb 13, 2021·Microorganisms·Cátia PacíficoQendrim Zebeli

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