Detachment of transformed cells. Role of CD44 variants

Cell Biophysics
C SantosK W Anderson

Abstract

A parallel-plate flow chamber was used to quantify the detachment of normal cloned rat embryo fibroblasts (CREF) fibroblasts, ras-transformed CREF fibroblasts (CREF T24), and CREF T24 fibroblasts transfected with a Krev/RAP1A suppressor gene (HK B1) from a confluent monolayer of normal CREF fibroblasts to determine if the expression patterns of CD44 variants (mol wt 110 and 140 kDa) corresponded with detachment properties and metastatic potential. In the detachment assay, known shear stresses ranging from 20-24 dyn/cm2 were applied to the adherent cells and the number of cells detached from the monolayer after 180 s was determined. Results showed that cellular expression of CD44 variants correlated with the metastatic potential of the cells and with the cells' ability to detach from a monolayer of normal cells. Western blot analysis showed a low level of expression of the CD44 variants in the normal cell line, CREF, and the lowly metastatic cell line, HK B1. Detachment studies showed a low percentage of detachment of both of these cell lines from a normal cell monolayer. Tumor-derived (HK B1-T) and lung nodule-derived (HK B1-M) cell lines were established and both formed tumors and metastasis with reduced latency periods as com...Continue Reading

References

Aug 1, 1992·The Journal of Cell Biology·L ThomasI Stamenkovic
Dec 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·G R ScreatonJ I Bell
Feb 20, 1992·International Journal of Cancer. Journal International Du Cancer·M C KuppnerN de Tribolet
Feb 1, 1992·The Journal of Cell Biology·S Jalkanen, M Jalkanen
Oct 1, 1991·The Journal of Experimental Medicine·M S SyI Stamenkovic
Oct 1, 1991·Molecular Immunology·B T BosworthJ A Harp
Jun 1, 1986·Immunological Reviews·S JalkanenE C Butcher
Jan 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M NodaY Ikawa
Dec 1, 1989·Immunology Today·B F HaynesS M Denning
Oct 1, 1989·Current Opinion in Cell Biology·G L Nicolson
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·L E BabissP B Fisher
Mar 14, 1986·Cell·M GallatinI L Weissman
Jan 1, 1988·Advances in Cancer Research·B T SherI L Weissman
Jan 1, 1983·Cancer Metastasis Reviews·L Weiss, P M Ward
Jun 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·P B FisherH S Ginsberg
Jan 1, 1994·The Journal of Cell Biology·C R MackayU Günthert
Jan 1, 1993·Advances in Immunology·J LesleyP W Kincade
Feb 1, 1993·The Journal of Experimental Medicine·S SeiterM Zöller
Feb 1, 1993·The Journal of Investigative Dermatology·L ThomasH R Byers
Apr 1, 1963·Experimental Cell Research·L WEISS, R R COOMBS
Jan 14, 1964·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·J L GOWANS, E J KNIGHT
Oct 1, 1964·Experimental Cell Research·L WEISS, P J LACHMANN

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Citations

May 20, 1999·Experimental and Molecular Pathology·A Herrera-Gayol, S Jothy
Jul 25, 2003·American Journal of Hematology·Paula S WalmetTimothy M Wick

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