Detailed Characterization of the Post-Ionization Efficiencies in MALDI-2 as a Function of Relevant Input Parameters

Journal of the American Society for Mass Spectrometry
Alexander PotthoffJ Soltwisch

Abstract

A recently introduced technique based on MALDI with laser-induced post-ionization (PI), also named MALDI-2, increases the ion yields for numerous classes of lipids, metabolites, and carbohydrates in MALDI-MS imaging experiments under certain experimental conditions. Here we used a semi-automatic LabVIEW-based protocol to investigate and optimize the efficiency of the PI process in dependence of four relevant input parameters and a dense parameter grid: pulse energies of the two lasers, delay between the laser pulses, and buffer gas pressure in the ion source. All experiments were conducted with a modified MALDI-2 Synapt G2-S mass spectrometer (Waters) and use of a focal spot size on the sample of 15-17 µm. A wavelength-tunable optical parametric oscillator (OPO) laser served for PI at 260 nm or 280 nm. The investigated MALDI matrices were: 2,5-dihydroxybenzoic acid (pos. ion mode, +), 2,5-dihydroxyacetophenone (+), α-cyano-4-hydroxycinnamic acid (+), norharmane (neg. ion mode, -), and 1,5-diaminonapthalene (-). A porcine brain extract served as lipid standard. In the positive ion mode, a maximum boost for the generated [M + H]+ species was found with a N2 buffer gas pressure of ~2 mbar and a delay between the laser emissions of...Continue Reading

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Citations

Mar 4, 2021·Journal of the American Society for Mass Spectrometry·Michael J TaylorChristopher R Anderton
Mar 30, 2021·Journal of the American Society for Mass Spectrometry·Eike U BrockmannKlaus Dreisewerd
Jun 16, 2021·Expert Review of Proteomics·Peggi M AngelRichard Drake
Sep 26, 2020·Analytical Chemistry·Bram HeijsKlaus Dreisewerd
Sep 19, 2020·Journal of the American Society for Mass Spectrometry·Marcel NiehausJosephine Bunch
Sep 14, 2021·Journal of the American Society for Mass Spectrometry·Josiah C McMillenRichard M Caprioli

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