Detailed kinetic performance analysis of micromachined radially elongated pillar array columns for liquid chromatography

Journal of Chromatography. a
Manly CallewaertWim De Malsche

Abstract

The individual factors that determine the kinetic performance (B- and C-term band broadening and bed permeability Kv) of radially elongated pillar (REP) columns are studied. To this end, columns with REPs having 4 different aspect ratios (AR=9, 12, 15, 20) were characterized experimentally and by means of numerical simulations. A tortuosity and retention based plate height equation was established, enabling a good global fit for all studied conditions. The B-term plate height contribution appears to decrease with a factor equaling the square of the flow path tortuosity τ. Going from AR=12 to AR=20 (τ=5.7 and τ=9.0 respectively), this resulted in a shift in plate height expressed in axial coordinates from Hmin=0.42μm to Hmin=0.25 for non-retained conditions and from H=0.77μm to H=0.57μm for a component with k=1.0. The obtained parameters were combined to predict optimal time-efficiency combinations for all possible channel lengths. This revealed an efficiency limit of N=10(7) plates for a non-retained component and N=7-8×10(6) for k=1 for a channel with an AR=20, corresponding to a channel length of 2.5m and a void time of 2.4h.

References

Jul 2, 2003·Journal of Chromatography. a·H Poppe
Sep 15, 2006·Analytical Chemistry·M De PraP J Schoenmakers
Nov 6, 2007·Analytical Chemistry·M BarrandeR Denoyel
Apr 7, 2009·Journal of Chromatography. a·Kanji MiyabeGeorges Guiochon
Feb 18, 2010·Analytical Chemistry·Douglas S MalkinMary J Wirth
Aug 21, 2013·Journal of Chromatography. a·Szabolcs Fekete, Davy Guillarme
Jun 16, 2015·Analytical Chemistry·Gert DesmetWim De Malsche

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Citations

Sep 10, 2020·Journal of Separation Science·Ken Broeckhoven, Gert Desmet
Jul 10, 2017·Journal of Chromatography. a·Shunta FutagamiWim De Malsche
Dec 11, 2020·Analytical Chemistry·K Broeckhoven, G Desmet

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