Detailed O-glycomics of the Muc2 mucin from colon of wild-type, core 1- and core 3-transferase-deficient mice highlights differences compared with human MUC2.

Glycobiology
Kristina A ThomssonGunnar C Hansson

Abstract

The heavily O-glycosylated mucin MUC2 constitutes the major protein in the mucosal layer that acts as a physical barrier protecting the epithelial layer in the colon. In this study, Muc2 was purified from mucosal scrapings from the colon of wild-type (WT) mice, core 3 transferase knockout (C3Gnt(-/-)) mice and intestinal epithelial cell-specific core 1 knockout (IEC C1Galt1(-/-)) mice. The Muc2 O-glycans were released by reductive β-elimination and analyzed with liquid chromatography-mass spectrometry in the negative-ion mode. Muc2 from the distal colon of WT and C3Gnt(-/-) knockout mice carried a mixture of core 1- or core 2-type glycans, whereas Muc2 from IEC C1Galt1(-/-) mice carried highly sialylated core 3- and core 4-type glycans. A large portion of NeuAc in all mouse models was positioned on disialylated N-acetyllactosamine units, an epitope not reported on human colonic MUC2. Mass spectra and proton NMR spectroscopy revealed an abundant NeuAc linked to internally positioned N-acetylglucosamine on colonic murine Muc2, which also differs markedly from human MUC2. Our results highlight that murine colonic Muc2 O-glycosylation is substantially different from human MUC2, which could be one explanation for the different comme...Continue Reading

References

Jan 1, 1989·Annual Review of Biochemistry·K A Karlsson
Sep 4, 2003·The Journal of Biological Chemistry·Catherine RobbeJean-Claude Michalski
Feb 18, 2004·Rapid Communications in Mass Spectrometry : RCM·Catherine RobbeJean-Claude Michalski
May 4, 2004·Journal of the American Society for Mass Spectrometry·Niclas G KarlssonNicolle H Packer
Sep 14, 2004·The Biochemical Journal·Catherine RobbeJean-Claude Michalski
Feb 11, 2005·International Journal of Cancer. Journal International Du Cancer·Seiichi SaitoYoichi Arai
Mar 10, 2005·Proceedings of the National Academy of Sciences of the United States of America·Toshie IwaiHisashi Narimatsu
Mar 26, 2005·Science·Fredrik BäckhedJeffrey I Gordon
Apr 9, 2005·Glycobiology·Kristina A ThomssonNiclas G Karlsson
Jul 22, 2005·Proceedings of the National Academy of Sciences of the United States of America·Ruth E LeyJeffrey I Gordon
Jan 16, 2007·Molecular & Cellular Proteomics : MCP·Ylva Andersch-BjörkmanGunnar C Hansson
May 23, 2007·The Journal of Experimental Medicine·Guangyu AnLijun Xia
Mar 4, 2008·Journal of Proteome Research·Alessio CeroniStuart M Haslam
May 8, 2008·Bioscience, Biotechnology, and Biochemistry·Shou Takashima
Sep 23, 2008·Proceedings of the National Academy of Sciences of the United States of America·Malin E V JohanssonGunnar C Hansson
Mar 12, 2009·Methods in Molecular Biology·Hasse KarlssonGunnar C Hansson
Apr 8, 2009·Molecular and Cellular Biology·Erica L StoneJamey D Marth
Mar 9, 2011·The Journal of Clinical Investigation·Jianxin FuLijun Xia
Nov 18, 2011·Trends in Microbiology·Nathalie Juge

❮ Previous
Next ❯

Citations

Mar 28, 2013·Chemical Reviews·William R AlleyMilos V Novotny
May 31, 2013·Glycobiology·Angela MarcobalJustin L Sonnenburg
Jun 12, 2013·Glycobiology·Kirk S B Bergstrom, Lijun Xia
Feb 8, 2014·American Journal of Physiology. Gastrointestinal and Liver Physiology·Olivia I KoberNathalie Juge
Oct 27, 2015·Nature Reviews. Microbiology·Gregory P DonaldsonSarkis K Mazmanian
Mar 22, 2014·Journal of the American Chemical Society·Xin MengHongzhi Cao
Jul 17, 2013·Proteomics. Clinical Applications·Tongzhong JuRichard D Cummings
Oct 31, 2012·The International Journal of Biochemistry & Cell Biology·Sumaira Z HasnainDavid J Thornton
Apr 4, 2015·Tissue Barriers·Steve CornickKris Chadee
May 27, 2015·Nutrition Reviews·Devon KavanaughRita M Hickey
Apr 10, 2014·Gut Microbes·Gráinne LennonP Ronan O'Connell
Apr 9, 2015·Frontiers in Genetics·Louise E TailfordNathalie Juge
Sep 30, 2016·HLA : Immune Response Genetics·C FuH Chen
Dec 25, 2016·Histochemistry and Cell Biology·Anthony Corfield
Jan 27, 2017·Molecular & Cellular Proteomics : MCP·Hannes HinneburgDaniel Kolarich
Jan 30, 2018·Microbial Biotechnology·Kees C H van der ArkClara Belzer
Dec 9, 2017·Frontiers in Microbiology·Muriel Mercier-Bonin, Marie-Pierre Chapot-Chartier
May 28, 2016·American Journal of Physiology. Gastrointestinal and Liver Physiology·Nan GaoLijun Xia
Sep 1, 2017·Immunological Reviews·Jeremy J Barr
Dec 12, 2017·Mass Spectrometry Reviews·Qiwei ZhangJianjun Li
Feb 7, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Charlotte LeclaireNathalie Juge
Jul 9, 2013·American Journal of Physiology. Gastrointestinal and Liver Physiology·Jessica M Holmén LarssonGunnar C Hansson
Jun 22, 2017·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·Hervé RobertIsabelle P Oswald
Nov 17, 2019·International Journal of Molecular Sciences·Zheng-Shun WenXin-Yan Han
Mar 11, 2020·Nature Microbiology·Gregory P DonaldsonSarkis K Mazmanian
Nov 11, 2017·American Journal of Physiology. Gastrointestinal and Liver Physiology·Joannie M AllaireBruce A Vallance
Apr 10, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Qianyun FuGuangli Yu
Jun 5, 2019·FEMS Microbiology Reviews·Lucie Etienne-MesminStéphanie Blanquet-Diot
Feb 23, 2019·Gastroenterology Report·Bjoern O Schroeder

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.