Detecting aerobic bacterial diversity in patients with diabetic foot wounds using ERIC-PCR: a preliminary communication

The International Journal of Lower Extremity Wounds
Surya K SinghSanjeev K Gupta

Abstract

The polymicrobial nature of diabetic foot infection is a reflection of the immune compromised state of the host.The methods of microbial identification based on colony morphology and biochemical characteristics have limitations as they may not differentiate the diverse microorganisms that infect foot wounds. The aim of the present study was to find out the bacterial diversity in diabetic foot infections at genetic level by finger printing, that is, ERIC-PCR (enterobacterial repetitive intergenic consensus -polymerase chain reaction). Nine patients with infected diabetic foot ulcers were recruited to the study. Pus and tissue samples were collected from the wound site. Aerobic bacteria were isolated employing standard microbiological culture methods and their genetic variability was analyzed using the ERIC-PCR. Sensitivity test for these isolates against commonly used antibiotics were performed using disc diffusion method. The standard microbiological culture technique yielded 38 morphotypes of bacteria and their genetic diversity was confirmed by ERIC-PCR assay. Analysis of the similarity index using NTSYSpc 2.1 software revealed 34 types of banding pattern among these isolates. Based on the similarity index these isolates were...Continue Reading

References

Oct 1, 1976·Annals of Internal Medicine·T J LouieS L Gorbach
Oct 1, 1992·Diabetic Medicine : a Journal of the British Diabetic Association·J A Hunt
Nov 7, 1996·The New England Journal of Medicine·H S Gold, R C Moellering
Jan 5, 2000·Journal of Clinical Microbiology·K Rantakokko-JalavaP Kotilainen
Jun 22, 2000·Molecular and Cellular Probes·R RedkarV DelVecchio
Mar 22, 2003·Diabetic Medicine : a Journal of the British Diabetic Association·Y GeK Holroyd
Sep 19, 2003·European Journal of Internal Medicine·F J. Candel GonzálezJ J. Picazo
May 4, 2005·Journal of Diabetes and Its Complications·Adel AbdulrazakLubna Ahmed Mobasher
Nov 30, 2005·Archives of Medical Research·Shankar Esaki MuthuUsha Anand Rao
Jul 29, 2006·Diabetes Care·Ravisekhar GadepalliRama Chaudhry
Oct 5, 2006·The Journal of Infection·Ozlem KandemirRamazan Gen
Feb 6, 2007·Clinics in Dermatology·Haris M Rathur, Andrew J M Boulton
May 3, 2007·Diabetes Care·Albert SottoUNKNOWN Nîmes University Hospital Working Group on the Diabetic Foot (GP30)
Apr 4, 2008·Diabetes/metabolism Research and Reviews·Jan Apelqvist

❮ Previous
Next ❯

Citations

May 24, 2012·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Benjamin A Lipsky Infectious Diseases Society of America
Jan 19, 2016·Frontiers in Microbiology·Shailesh K Shahi, Ashok Kumar
Jan 28, 2016·Diabetes/metabolism Research and Reviews·Edgar J Peters
Sep 20, 2015·Diabetes/metabolism Research and Reviews·Benjamin A LipskyEdgar J G Peters
Oct 1, 2011·Diabetes & Metabolic Syndrome·Shalbha TiwariSurya K Singh
Nov 1, 2015·Revista Da Sociedade Brasileira De Medicina Tropical·Michele Cezimbra PerimMaria Cristina da Silva Pranchevicius
May 4, 2013·Journal of Medical Systems·Farid Touati, Rohan Tabish

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Related Papers

Diabetes Care
American Diabetes Association
The New England Journal of Medicine
Edward AbrahamAdministration of Drotrecogin Alfa (Activated) in Early Stage Severe Sepsis (ADDRESS) Study Group
© 2022 Meta ULC. All rights reserved