PMID: 9177164Jun 10, 1997Paper

Detecting and characterizing N-acyl-homoserine lactone signal molecules by thin-layer chromatography

Proceedings of the National Academy of Sciences of the United States of America
P D ShawS K Farrand

Abstract

Many Gram-negative bacteria regulate gene expression in response to their population size by sensing the level of acyl-homoserine lactone signal molecules which they produce and liberate to the environment. We have developed an assay for these signals that couples separation by thin-layer chromatography with detection using Agrobacterium tumefaciens harboring lacZ fused to a gene that is regulated by autoinduction. With the exception of N-butanoyl-L-homoserine lactone, the reporter detected acyl-homoserine lactones with 3-oxo-, 3-hydroxy-, and 3-unsubstituted side chains of all lengths tested. The intensity of the response was proportional to the amount of the signal molecule chromatographed. Each of the 3-oxo- and the 3-unsubstituted derivatives migrated with a unique mobility. Using the assay, we showed that some bacteria produce as many as five detectable signal molecules. Structures could be assigned tentatively on the basis of mobility and spot shape. The dominant species produced by Pseudomonas syringae pv. tabaci chromatographed with the properties of N-(3-oxohexanoyl)-L-homoserine lactone, a structure that was confirmed by mass spectrometry. An isolate of Pseudomonas fluorescens produced five detectable species, three o...Continue Reading

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Citations

Dec 1, 2006·Archives of Microbiology·Stéphane UrozYves Dessaux
Aug 6, 2005·Analytical and Bioanalytical Chemistry·Peter J SchuppPeter D Steinberg
Apr 15, 2008·Analytical and Bioanalytical Chemistry·Anjali KumariSylvia Daunert
Jan 12, 2010·Analytical and Bioanalytical Chemistry·Jian-Hua WangSheng-Di Fan
Jul 30, 2010·Analytical and Bioanalytical Chemistry·Xiao ChenPetra M Krämer
Mar 14, 2007·Microbial Ecology·Torben MartensThorsten Brinkhoff
Aug 5, 2010·Applied Microbiology and Biotechnology·Ting-ting RenXiao-yan Li
Jan 7, 2011·Applied Microbiology and Biotechnology·Sayani MitraJoydeep Mukherjee
Feb 15, 2011·Applied Microbiology and Biotechnology·Joanne HothersallChristopher M Thomas
Sep 1, 2005·Current Microbiology·Neelawan PongsilpMichael J Sadowsky
Sep 4, 2009·Current Microbiology·Jishun LuYuquan Xu
Dec 18, 2010·Molecular Genetics and Genomics : MGG·Eiko KandaYuki Ichinose
May 24, 2005·Extremophiles : Life Under Extreme Conditions·Inmaculada LlamasJuan E González
Dec 27, 2011·Folia Microbiologica·Dorota StankowskaWieslaw Kaca
Sep 12, 2002·International Journal of Food Microbiology·Lone GramMichael Givskov
Aug 29, 2001·FEMS Microbiology Reviews·N A WhiteheadG P Salmond
Dec 4, 2010·Chemical Reviews·Mair E A Churchill, Lingling Chen
Sep 29, 2011·Journal of Agricultural and Food Chemistry·Shanmugam N PrashanthNicola S Iacobellis
Feb 28, 2006·Nature Reviews. Microbiology·Laurent Keller, Michael G Surette

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