PMID: 8606793Nov 1, 1995Paper

Detecting changes in calcium influx which contribute to synaptic modulation in mammalian brain slice

Neuropharmacology
B L Sabatini, W G Regehr

Abstract

The control of neurotransmitter release by modulation of presynaptic calcium influx was investigated at the granule cell to Purkinje cell synapse in rat cerebellar slices. Excitatory post-synaptic currents were measured using whole cell voltage clamp, and changes in presynaptic Ca influx were determined with the Ca-sensitive dye mag-fura-5. Single stimuli of the parallel fibers evoked rapid changes in mag-fura-5 fluorescence which increased from 10 to 90% in 1.4 msec, and then decayed within hundreds of milliseconds to prestimulus levels. These fluorescence changes were unaffected by disruption of internal stores with ryanodine or thapsigargin, and were reduced by 79% by the calcium channel toxin omega-conotoxin-MVIIC. We conclude that these signals result from calcium entry into presynaptic terminals through voltage gated calcium channels opened by action potentials. These fluorescence signals allow us to quantitate changes in calcium influx. We used this approach to study the enhancement of stimulus-evoked synaptic currents by 3-isobutyl-1-methylxanthine (IBMX), a phosphodiesterase inhibitor and antagonist of adenosine receptors. Both enhancement of calcium influx into presynaptic terminals, and reduction in the firing thresh...Continue Reading

References

May 1, 1992·The Journal of Physiology·E Neher, G J Augustine
Oct 1, 1992·The Journal of Physiology·A J IrvingJ G Schofield
Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·A KonnerthC M Armstrong
Sep 1, 1987·The American Journal of Physiology·E RousseauG Meissner
Mar 1, 1989·The American Journal of Physiology·B RajuR E London
Feb 28, 1985·Biochimica Et Biophysica Acta·J J Feher, G B Lipford
Nov 1, 1974·Journal of Neurocytology·V Chan-PalayS M Billings-Gagliardi
Aug 1, 1974·Journal of Neurocytology·R F Foelix, R Oppenheim
Apr 1, 1967·The Journal of Physiology·B Katz, R Miledi
May 1, 1995·Biophysical Journal·W G Regehr, P P Atluri
Dec 1, 1993·The Journal of Physiology·S J SmithG J Augustine
Feb 1, 1994·Cell Calcium·H IatridouH E Katerinopoulos
Apr 1, 1993·The Journal of Physiology·O Delbono, E Stefani
Jan 18, 1994·Proceedings of the National Academy of Sciences of the United States of America·K R DelaneyD Kleinfeld

❮ Previous
Next ❯

Citations

Mar 25, 1998·Biophysical Journal·B L Sabatini, W G Regehr
Mar 30, 1999·Biophysical Journal·M A Xu-Friedman, W G Regehr
Aug 2, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Jennifer D WarnerDavid R Giovannucci
Sep 26, 2008·Journal of Neurophysiology·Evan S HillPaul S Katz
Apr 8, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Michael H Myoga, Wade G Regehr
Jan 8, 2016·Nature·Skyler L JackmanWade G Regehr
Nov 14, 1996·Nature·B L Sabatini, W G Regehr
Jul 6, 2014·Cold Spring Harbor Protocols·Stephan D Brenowitz, Wade G Regehr

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.