Detecting clonal rearrangement in non-Hodgkin's lymphomas in Taiwan by polymerase chain reaction

Leukemia & Lymphoma
Shih-Sung ChuangWenya Huang

Abstract

The detection of monoclonal expansions of the immunoglobulin heavy chain (IgH) or the T-cell receptor-gamma (TCRgamma) chain genes is an important supplement for the diagnosis of the non-Hodgkin's lymphomas (NHLs). Detection of monoclonality by polymerase chain reaction (PCR) method has offered an efficient approach for rapid diagnosis and monitoring of the therapeutic effects. Here we conducted a retrospective PCR clonality study on 49 cases of NHLs including 23 B-cell lymphomas (BCLs), 20 peripheral T-cell lymphomas (PTCLs), 6 natural killer (NK)/T-cell lymphomas and 3 reactive lymphoid tissues from southern Taiwan. Genomic DNAs from paraffin sections were extracted and analyzed by the IgH- and TCR-specific PCR reactions. The results showed that 20 of 23 (87.5%) BCLs exhibited IgH gene rearrangements and were all germline for TCRgamma. 15 of 20 (75.0%) PTCLs exhibited TCRgamma gene rearrangements while 1 case (5%) was positive for IgH gene rearrangement. The 6 NK/T-cell lymphomas and 3 reactive lymphoid tissues were all germline for either IgH or TCRgamma genes. Our results were similar to other Western reports in terms of sensitivity and cell-lineage specificity. This is the first large series of PCR clonality study of IgH a...Continue Reading

Citations

Jan 22, 2011·The American Journal of Dermatopathology·Shien-Tung PanShih-Sung Chuang
Jun 30, 2009·The American Journal of Surgical Pathology·Shih-Sung ChuangHongxiang Liu
Sep 1, 2007·Journal of Clinical Pathology·Shih-Sung ChuangPeter G Isaacson
Mar 26, 2016·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Mineui HongYoung-Hyeh Ko
Jul 25, 2009·Pathology, Research and Practice·Sheng-Tsung ChangShih-Sung Chuang

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