Detecting cytokeratin 19 mRNA in the peripheral blood cells of cervical cancer patients and its clinical-pathological correlation

Gynecologic Oncology
Chiou-Chung YuanWen-Yuann Shyong

Abstract

The aim of this study was to study the presence of cytokeratin 19 (CK19)-expressing cancer cells in the blood of preoperative patients with FIGO stage Ib and IIb cervical cancers who received radical hysterectomy and to investigate the cells' clinical significance. CK19 mRNA in the blood cells of the patients was detected preoperatively by a newly designed nested reverse transcriptase-polymerase chain reaction, which excluded pseudogenes a and b, performed on 84 patients with stage Ib and IIb cervical carcinoma. Possible correlations between clinicopathological factors were then analyzed. The sensitivity of this assay was 1 CK19-mRNA-positive cell per 10(6) peripheral blood mononuclear cells. Results showed that 21.4% of the 84 patients with cervical carcinoma had CK19-mRNA-positive cells in the blood, in comparison with 5.7% of the 35 patients with benign gynecological tumors and 0% of the 28 healthy controls (P = 0.037 and 0.006, respectively). The positive tests in the cervical cancer patients were not associated with prognostic factors including stage, pelvic lymph node metastasis, pathological types, bulky tumor size (> or =4 cm), differentiation, parametrial extension, lymphovascular space involvement, deep stromal invasi...Continue Reading

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Citations

May 10, 2006·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·H Y WangS Q Fu
Sep 9, 2006·Clinical Chemistry and Laboratory Medicine : CCLM·Ina H BenoyLuc Y Dirix
Jul 12, 2005·Cancer Letters·Shung-Haur YangChi-Jung Huang
Aug 8, 2006·Gynecologic Oncology·T FehmE F Solomayer
Aug 2, 2011·International Journal of Gynecological Pathology : Official Journal of the International Society of Gynecological Pathologists·Colin J R StewartKathryn Louwen
Aug 27, 2018·Virchows Archiv : an International Journal of Pathology·Ben Davidson
Aug 28, 2021·Cancers·Paola CafforioStella D'Oronzo

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