Detecting Dengue Virus Nonstructural Protein 1 (NS1) in Urine Samples Using ELISA for the Diagnosis of Dengue Virus Infection

Japanese Journal of Infectious Diseases
Yuka SaitoTomohiko Takasaki

Abstract

Dengue virus (DENV) infection is a serious global health threat. For the surveillance and control of dengue, there is a need for robust diagnostic tools that are relatively easy to use and reliable in various clinical settings. We investigated the applicability of NS1 antigen detection in urine samples for the diagnosis of DENV. About 118 urine samples, obtained from 96 dengue patients at various phases of disease, were used for this study. NS1 antigen was detected by ELISA in the urine samples obtained from patients after 2-17 days of disease onset. Positive detection rates of NS1 antigen ranged between 13-43%. Based on real-time RT-PCR, positive detection rates of viral genome in the urine samples ranged between 20-33% on days 0 to ≥15. On days 11 to ≥15 after the disease onset, NS1 antigen was detected at similar rates in serum and urine samples. Additionally, NS1 antigen was detected in 2 urine samples, but not in the serum samples, on days 7 and 16 after the onset of the disease. The results confirm the applicability of NS1 antigen detection in urine samples using ELISA to diagnose acute DENV infection and suggests that the assay is potentially useful when only limited amounts of serum samples are available and in limited ...Continue Reading

References

Jul 11, 2006·Lancet·Jacqueline L DeenJeremy J Farrar
Aug 18, 2006·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·S VázquezM G Guzmán
Apr 10, 2007·Transactions of the Royal Society of Tropical Medicine and Hygiene·Y MizunoT Takasaki
Apr 21, 2010·PLoS Neglected Tropical Diseases·Tran Nguyen Bich ChauCameron P Simmons
Mar 18, 2011·Journal of Applied Microbiology·J WuM D Sobsey
Apr 27, 2011·Proceedings of the National Academy of Sciences of the United States of America·Irina GutscheMarie Flamand
Apr 13, 2012·The New England Journal of Medicine·Cameron P SimmonsBridget Wills
May 26, 2012·PLoS Neglected Tropical Diseases·Vanessa RaclozWenbiao Hu
Apr 9, 2013·Nature·Samir BhattSimon I Hay
Aug 31, 2013·Clinical Epidemiology·Natasha Evelyn Anne MurrayAnnelies Wilder-Smith
Apr 3, 2014·Current Tropical Medicine Reports·Alexander W E FranzA Lorena Passarelli
Sep 23, 2014·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Essi M KorhonenOlli Vapalahti

❮ Previous
Next ❯

Citations

Sep 26, 2015·PLoS Neglected Tropical Diseases·Anne-Claire AndriesPhilippe Buchy
Sep 29, 2017·Science Translational Medicine·Irene BoschLee Gehrke
Jul 12, 2016·Tropical Medicine and Health·Meng Ling MoiIchiro Kurane

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.