PMID: 6164773Nov 1, 1980Paper

Detecting dentinal sclerosis in decalcified sections with the Pollak trichrome connective tissue stain

Journal of Oral Pathology
H R StanleyM Schultz

Abstract

The supply of young human teeth for controlled human pulp studies is inadequate. Dentinal sclerosis in older teeth modifies the expected pulpal responses. Decalcification, necessary to interpret pulpal responses, destroys the primary evidence of sclerosis. At present we are unable to detect the degree of sclerosis and the pulp response in the same preparation. Valid interpretations on older teeth cannot be accomplished until this problem is resolved. Fifty-seven teeth, normal and carious, representing a wide age span were fixed, embedded in Bioplastic and sectioned with the Bronwill Model No. 77 through the middle of the M-D plane on the labio-lingual plane to obtain an undecalcified ground section for examination with reflected and transmitted light and Faxitron 805 microradiography. The remaining portions of the tooth were decalcified, processed in paraffin and subjected to a variety of histochemical reactions including the Pollak trichrome stain. The Pollak trichrome and the Pollak trichrome solution No. 6 modification stains provided the greatest correlations with the sclerotic areas in the radiographs of the ground sections. Areas of dentin indicative of sclerosis prior to decalcification stained orange-red with the Pollak...Continue Reading

References

Oct 1, 1975·The Journal of the American Dental Association·H R StanleyH H Chauncey
Dec 1, 1966·The Journal of Nihon University School of Dentistry·S IsokawaH Honda
Apr 1, 1968·Oral Surgery, Oral Medicine, and Oral Pathology·H R Stanley
Apr 1, 1967·Archivum Histologicum Japonicum = Nihon Soshikigaku Kiroku·T Shimoyama
Aug 1, 1958·Journal of Dental Research·R G CRAIG, F A PEYTON
May 1, 1959·Journal of Dental Research·R G CRAIGF A PEYTON
Apr 27, 1955·Annals of the New York Academy of Sciences·R F SOGNNAES
Jan 1, 1952·Oral Surgery, Oral Medicine, and Oral Pathology·F R SHROFF

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Citations

Nov 1, 1989·Oral Surgery, Oral Medicine, and Oral Pathology·H R Stanley
Jan 1, 1990·The Journal of the American Dental Association·H R Stanley

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