Detecting food- and waterborne viruses by surface-enhanced Raman spectroscopy.

Journal of Food Science
Cui FanMengshi Lin

Abstract

Food- and waterborne viruses pose serious health risks to humans and were associated with many outbreaks worldwide. Rapid, accurate, and nondestructive methods for detection of viruses are of great importance to protect public health. In this study, surface-enhanced Raman spectroscopy (SERS) coupled with gold SERS-active substrates was used to detect and discriminate 7 food- and waterborne viruses, including norovirus, adenovirus, parvovirus, rotavirus, coronavirus, paramyxovirus, and herpersvirus. Virus samples were purified and dialyzed in phosphate buffered saline (8 to 9 log PFU/mL) and then further diluted in deionized water for SERS measurement. After capturing the characteristic SERS spectral patterns, multivariate statistical analyses, including soft independent modeling of class analogy (SIMCA) and principal component analysis (PCA), were employed to analyze SERS spectral data for characterization and identification of viruses. The results show that SIMCA was able to differentiate viruses with and without envelope with >95% of classification accuracy, while PCA presented clear spectral data segregations between different virus strains. The virus detection limit by SERS using gold substrates reached a titer of 10(2).

References

Jun 19, 2002·FEMS Microbiology Reviews·Marion KoopmansSteve Monroe
Jan 28, 2003·Critical Reviews in Microbiology·H LeclercE Dei-Cas
Feb 24, 2007·Analytical and Bioanalytical Chemistry·Bin RenZhong-Qun Tian
Jun 3, 2009·Journal of Applied Microbiology·R G SinclairC P Gerba
Jul 19, 2008·Annual Review of Analytical Chemistry·Paul L StilesRichard P Van Duyne

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Citations

Apr 12, 2012·Journal of Food Science·Huirong TangYiping Du
Mar 14, 2020·Foods·Lingling Liu, Matthew D Moore
Oct 23, 2019·Journal of Agricultural and Food Chemistry·Andrea BernatQinchun Rao

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Methods Mentioned

BETA
electron microscopy
Raman
chip
PCA
atomic force microscopy

Software Mentioned

SIMCA
Delight
WiRE

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