Detecting genetic variability among different Mycobacterium tuberculosis strains using DNA microarrays technology

Tuberculosis
Raul DiazEric J Rubin

Abstract

Recent advances in functional and comparative genomics have improved our understanding of genetic diversity among the Mycobacterium tuberculosis complex. In this study, we investigated the genetic polymorphism of M. tuberculosis using whole-genome microarray analysis. Amplified fragments of 15 M. tuberculosis strains (from two different geographical origins) and the reference strain H37Rv were produced by random amplification of polymorphic DNA (RAPD) using three different primers. The RAPD products were labeled with fluorescent dyes (Cy3 and Cy5) and hybridized to a TB DNA microarray representing nearly all open reading frames (ORFs) of H37Rv. The final results were analyzed using bioinformatic tools. Some genetic variability was found among the 16 M. tuberculosis strains. The majority of the highly polymorphic DNA sequences were observed in ORFs representing non-essential genes of the bacterium. The future use of comparative genomics based on DNA microarray technology should prove a powerful tool for understanding phenotypic variability among M. tuberculosis isolates of similar genetic composition. It is also a promising approach to provide important insights into evolution, virulence and pathogenesis of M. tuberculosis.

References

Mar 10, 2001·The Journal of Clinical Investigation·M Kato-MaedaP M Small
Oct 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·C M SassettiE J Rubin
Sep 10, 2002·Journal of Bacteriology·R D FleischmannC M Fraser
Mar 27, 2003·Molecular Microbiology·Christopher M SassettiEric J Rubin
Oct 22, 2003·Proceedings of the National Academy of Sciences of the United States of America·Christopher M Sassetti, Eric J Rubin
Mar 17, 2004·Proceedings of the National Academy of Sciences of the United States of America·Anthony G TsolakiPeter M Small

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Citations

Sep 6, 2008·Canadian Journal of Microbiology·G Vázquez-MarrufoM S Vázquez-Garcidueñas
Jan 1, 2013·Infectious Diseases·Balkis A TalipWilliam J Snelling
Dec 13, 2007·Veterinary Research·Shivani Ojha, Magdalena Kostrzynska

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