Detecting host-parasitoid interactions in an invasive Lepidopteran using nested tagging DNA metabarcoding

Molecular Ecology
James J N KitsonDavid H Lunt

Abstract

Determining the host-parasitoid interactions and parasitism rates for invasive species entering novel environments is an important first step in assessing potential routes for biocontrol and integrated pest management. Conventional insect rearing techniques followed by taxonomic identification are widely used to obtain such data, but this can be time-consuming and prone to biases. Here, we present a next-generation sequencing approach for use in ecological studies which allows for individual-level metadata tracking of large numbers of invertebrate samples through the use of hierarchically organised molecular identification tags. We demonstrate its utility using a sample data set examining both species identity and levels of parasitism in late larval stages of the oak processionary moth (Thaumetopoea processionea-Linn. 1758), an invasive species recently established in the United Kingdom. Overall, we find that there are two main species exploiting the late larval stages of oak processionary moth in the United Kingdom with the main parasitoid (Carcelia iliaca-Ratzeburg, 1840) parasitising 45.7% of caterpillars, while a rare secondary parasitoid (Compsilura concinnata-Meigen, 1824) was also detected in 0.4% of caterpillars. Using ...Continue Reading

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Citations

Oct 23, 2018·Molecular Ecology·Stephane A P DeroclesDarren M Evans
May 23, 2020·Journal of Fish Biology·Michael TraugottDaniela Sint
Jan 10, 2020·Molecular Ecology·Loren RiesebergPierre Taberlet
Jan 2, 2020·Evolutionary Applications·Richard C Hamelin, Amanda D Roe
May 2, 2020·Insects·Jetske G de Boer, Jeffrey A Harvey
May 26, 2021·Trends in Parasitology·Kirsten E MillerDarren M Evans
Sep 23, 2021·Molecular Ecology Resources·Kristine BohmannSimon Creer
Jan 15, 2022·Scientific Reports·Kasun H BodawattaKaterina Sam

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