Nested metabarcode tagging: a robust tool for studying species interactions in ecology and evolution

BioRxiv : the Preprint Server for Biology
James J N KitsonDavid H Lunt


Here we present a next generation sequencing approach for use in ecological studies which allows for individual level sample tracking through the use of hierarchically organised molecular identification tags. We demonstrate its utility on a sample data set examining levels of parasitism in a recently arrived invasive species in the UK and discuss further possibilities for our approach in ecology and evolution.

Related Concepts

Massively-Parallel Sequencing
Moth antigen
Insect Extract
Parasitic Diseases

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