DOI: 10.1101/482166Nov 29, 2018Paper

Detecting HTS Barcode Contamination

BioRxiv : the Preprint Server for Biology
Mallory A ClarkDarrell O Ricke

Abstract

DNA barcoding enables multiple samples to be characterized in parallel with high throughput sequencing (HTS) experiments for cost efficiencies. Cross-contamination of DNA barcode reagents can result in the detection of HTS sequences for barcodes that were not originally added to a particular sample. Cross-contamination of data between multiplexed samples can also occur. Avoidance and detection of contaminated barcodes is relevant for DNA forensic samples analysis, accurate cancer diagnosis, clinical research applications, metagenomic analysis, etc. We present recommendations for the avoidance of contamination and a tool, TallyBarcodes, to aid in the detection of DNA barcode contamination.

Related Concepts

Clinical Research
DNA
DNA Repair
Cancer Diagnosis
Analysis
DNA Barcoding, Taxonomic
DNA Barcode, Taxonomic
High-Throughput RNA Sequencing

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