Detecting interferon-gamma release from human CD4 T-cells using surface plasmon resonance

Colloids and Surfaces. B, Biointerfaces
Gulnaz StybayevaAlexander Revzin

Abstract

Cytokine secretion by leukocytes is an important indicator of immune response to pathogens and therefore has significant implications in disease diagnostics. Given heterogeneity of leukocyte subsets and the ability of multiple cell subsets to secrete the same cytokines, connecting cytokine production to a specific leukocyte subset is a distinct challenge. In the present paper we describe a strategy combining antibody (Ab)-based affinity cell separation and surface plasmon resonance (SPR) for capturing human CD4 T-cells and for label-free detection of cell-secreted interferon (IFN)-gamma--an important inflammatory cytokine. Human blood was introduced into a flow chamber modified with anti-CD4 Abs resulting in capture of CD4(+) T-cells. After mitogenic activation of cells inside the flow chamber, culture medium was routed onto an SPR chip modified with monoclonal IFN-gamma Abs. SPR signal observed in this experiment correlated with cytokine production by T-cells. The strategy of combining SPR detection with cell purification may be used in the future for label-free, sensitive detection of multiple cytokines or proteins secreted by the desired cell subset.

References

May 3, 2002·Nature·Daniel C DouekRichard A Koup
Jul 25, 2003·Analytical and Bioanalytical Chemistry·Jirí Homola
Jul 11, 2006·Journal of Immunological Methods·Kazuhiko SekineMehmet Toner
Jan 8, 2008·Electroanalysis·Jonathan S Daniels, Nader Pourmand
Nov 22, 2008·Lab on a Chip·He ZhuAlexander Revzin

❮ Previous
Next ❯

Citations

Jun 23, 2012·Analytical and Bioanalytical Chemistry·Ying LiuAleksandr L Simonian
Jun 2, 2012·Biomicrofluidics·Alexander RevzinH-C Chang
Dec 28, 2011·Methods : a Companion to Methods in Enzymology·Sarah MilgramYoann Roupioz
Oct 22, 2010·Biosensors & Bioelectronics·Sarah MilgramYoann Roupioz
Aug 15, 2015·Annual Review of Biomedical Engineering·Qing ZhouAlexander Revzin
Jun 17, 2014·Angewandte Chemie·Dong-Sik ShinAlexander Revzin
Apr 4, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Sunmin AhnM Selim Ünlü
Mar 23, 2011·Analytical Chemistry·Yinxi HuangPeng Chen
Oct 26, 2016·Colloids and Surfaces. B, Biointerfaces·Evan A DubielPatrick Vermette
Jul 16, 2013·Chemical Society Reviews·Jeffrey KirschAleksandr Simonian
Jun 12, 2021·Advanced Science·Chao LiuGuozhen Liu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Babesiosis

Babesiosis is caused by parasites of the genus babesia, which are transmitted in nature by the bite of an infected tick. Discover the latest research on babesiosis here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.