Detecting molecular interactions that stabilize native bovine rhodopsin

Journal of Molecular Biology
K Tanuj SapraK Palczewski

Abstract

Using single-molecule force spectroscopy we probed molecular interactions within native bovine rhodopsin and discovered structural segments of well-defined mechanical stability. Highly conserved residues among G protein-coupled receptors were located at the interior of individual structural segments, suggesting a dual role for these segments in rhodopsin. Firstly, structural segments stabilize secondary structure elements of the native protein, and secondly, they position and hold the highly conserved residues at functionally important environments. Two main classes of force curves were observed. One class corresponded to the unfolding of rhodopsin with the highly conserved Cys110-Cys187 disulfide bond remaining intact and the other class corresponded to the unfolding of the entire rhodopsin polypeptide chain. In the absence of the Cys110-Cys187 bond, the nature of certain molecular interactions within folded rhodopsin was altered. These changes highlight the structural importance of this disulfide bond and may form the basis of dysfunctions associated with its absence.

References

Jun 23, 1992·Biochemistry·K WakamatsuT Higashijima
Jul 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·T DoiH G Khorana
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·B KönigK P Hofmann
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·S S KarnikH G Khorana
Jan 1, 1982·Methods in Enzymology·D S Papermaster
Jan 1, 1995·Human Molecular Genetics·T P Dryja, T Li
May 14, 1996·Proceedings of the National Academy of Sciences of the United States of America·X LiuH G Khorana
Oct 6, 1997·Proceedings of the National Academy of Sciences of the United States of America·J HwaH G Khorana
Mar 7, 1998·Biochemistry·M KonoD D Oprian
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·J HwaH G Khorana
Mar 12, 1999·Science·M GrandboisH E Gaub
May 8, 1995·Current Opinion in Ophthalmology·M X WangL A Donoso
Feb 26, 2000·Annual Review of Genetics·A RattnerJ Nathans
Feb 7, 2001·Science·F OesterheltD J Müller
Jun 17, 2000·Trends in Endocrinology and Metabolism : TEM·A SalahpourM Bouvier
Aug 5, 2000·Science·K PalczewskiM Miyano
Aug 19, 2000·Science·H R Bourne, E C Meng
Apr 26, 2001·Proceedings of the National Academy of Sciences of the United States of America·J HwaH G Khorana
Jun 8, 2001·Proteins·D J JacobsM F Thorpe
Dec 10, 2002·Annual Review of Physiology·Sławomir FilipekKrzysztof Palczewski
Jan 10, 2003·Nature·Dimitrios FotiadisKrzysztof Palczewski
Feb 8, 2003·Annual Review of Biophysics and Biomolecular Structure·Slawomir FilipekRonald Stenkamp
Mar 12, 2003·Biochemistry·Tara MirzadeganKrzysztof Palczewski
Mar 29, 2003·The Journal of Biological Chemistry·Yan LiangAndreas Engel
Jun 5, 2003·Journal of Structural Biology·Clemens MöllerDaniel J Müller
Sep 19, 2003·Trends in Biochemical Sciences·Kevin D RidgeKrzysztof Palczewski
Oct 1, 2003·The EMBO Journal·Harald JanovjakDaniel J Müller
Nov 25, 2003·FEBS Letters·Donald M EngelmanJean-Luc Popot
Apr 28, 2004·FEBS Letters·Dimitrios FotiadisKrzysztof Palczewski
May 5, 2004·Proceedings of the National Academy of Sciences of the United States of America·A J RaderJudith Klein-Seetharaman
Jul 9, 2004·Journal of Molecular Biology·Alexej KedrovDaniel J Müller
Aug 26, 2004·Journal of Molecular Biology·Tetsuji OkadaVolker Buss
Oct 20, 2004·Journal of Molecular Biology·Jade LiGebhard F X Schertler
Dec 3, 2004·Biophysical Journal·Harald JanovjakAndrew D L Humphris
Dec 15, 2004·Biochemistry·Paul S-H ParkKrzysztof Palczewski
Jun 16, 2005·The FEBS Journal·Graeme Milligan, Michel Bouvier
Jun 16, 2005·Proceedings of the National Academy of Sciences of the United States of America·Paul S-H Park, Krzysztof Palczewski
Jun 18, 2005·EMBO Reports·Alexej KedrovDaniel J Muller
Nov 22, 2005·Journal of Molecular Biology·Alexej KedrovDaniel J Muller

❮ Previous
Next ❯

Citations

May 15, 2007·Applied Microbiology and Biotechnology·Ana J García-Sáez, Petra Schwille
Nov 3, 2010·Biochemistry·Shiho KawamuraPaul S-H Park
Apr 8, 2009·Biochemistry·Paul S-H ParkKrzysztof Palczewski
Feb 13, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·K Tanuj SapraDaniel J Muller
Oct 12, 2011·Proceedings of the National Academy of Sciences of the United States of America·Lin GeDaniel J Muller
May 8, 2009·The Journal of Biological Chemistry·Christian A BippesDimitrios Fotiadis
Feb 17, 2007·The Journal of Biological Chemistry·Paul S-H ParkDaniel J Muller
May 24, 2007·Journal of the Royal Society, Interface·Ashok A DenizEdward A Lemke
Feb 5, 2009·Photochemistry and Photobiology·Craig McKibbinPaula J Booth
Mar 7, 2008·Pharmacological Reviews·Daniel J MüllerKrzysztof Palczewski
Feb 22, 2007·Annual Review of Biophysics and Biomolecular Structure·Alexej KedrovDaniel J Müller
May 31, 2007·The Journal of Cell Biology·Stephan NickellKrzysztof Palczewski
Apr 19, 2011·Trends in Pharmacological Sciences·Thomas Huber, Thomas P Sakmar
Oct 12, 2014·Biochimica Et Biophysica Acta·Allison M Whited, Paul S-H Park
Mar 29, 2006·Current Opinion in Structural Biology·Dimitrios FotiadisAndreas Engel
Apr 1, 2014·Current Opinion in Cell Biology·Tivadar OrbanKrzysztof Palczewski
Aug 7, 2008·Structure·David A CisnerosDaniel J Muller
Jun 27, 2012·Peptides·Michael S ParkerSteven L Parker
Jan 15, 2008·Journal of Molecular Biology·K Tanuj SapraDaniel J Muller
Jan 21, 2012·Biophysical Journal·Arfaxad Reyes-AlcarazPere Garriga
Oct 3, 2006·Biophysical Journal·Ana Vitória BotelhoMichael F Brown
Jun 27, 2006·Current Opinion in Structural Biology·Daniel J MüllerAndreas Engel
Mar 19, 2008·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Harald JanovjakDaniel J Müller
Apr 17, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Francesca Fanelli, Michele Seeber
Jun 27, 2013·Chemical Society Reviews·Michael ZocherDaniel J Müller
Feb 17, 2021·Pflügers Archiv : European journal of physiology·Paul S-H Park

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

The Journal of Biological Chemistry
Paul S-H ParkDaniel J Muller
Annual Review of Biophysics and Biomolecular Structure
Alexej KedrovDaniel J Muller
© 2021 Meta ULC. All rights reserved