Detecting neuroinflammation in the brain following chronic alcohol exposure in rats: A comparison between in vivo and in vitro TSPO radioligand binding

The European Journal of Neuroscience
Ryan TylerNora D Volkow

Abstract

Excessive alcohol consumption is associated with neuroinflammation, which likely contributes to alcohol-related pathology. However, positron emission tomography (PET) studies using radioligands for the 18-kDa translocator protein (TSPO), which is considered a biomarker of neuroinflammation, reported decreased binding in alcohol use disorder (AUD) participants compared to controls. In contrast, autoradiographic findings in alcohol exposed rats reported increases in TSPO radioligand binding. To assess if these discrepancies reflected differences between in vitro and in vivo methodologies, we compared in vitro autoradiography (using [3 H]PBR28 and [3 H]PK11195) with in vivo PET (using [11 C]PBR28) in male, Wistar rats exposed to chronic alcohol-vapor (dependent n = 10) and in rats exposed to air-vapor (nondependent n = 10). PET scans were obtained with [11 C]PBR28, after which rats were euthanized and the brains were harvested for autoradiography with [3 H]PBR28 and [3 H]PK11195 (n = 7 dependent and n = 7 nondependent), and binding quantified in hippocampus, thalamus, and parietal cortex. Autoradiography revealed significantly higher binding in alcohol-dependent rats for both radioligands in thalamus and hippocampus (trend level f...Continue Reading

References

Aug 1, 1992·The American Journal of Psychiatry·N D VolkowA P Wolf
Dec 1, 1988·Annals of Neurology·J BenavidesB Scatton
Apr 1, 1987·Biochemical Pharmacology·R TeschkeG Goerz
Apr 1, 1980·European Journal of Clinical Investigation·K E McCollA Goldberg
Dec 1, 1981·European Journal of Clinical Investigation·K E McCollA Goldberg
Aug 1, 1996·Trends in Neurosciences·G W Kreutzberg
May 1, 1997·Alcoholism, Clinical and Experimental Research·A PfefferbaumK O Lim
Nov 5, 1997·Alcoholism, Clinical and Experimental Research·N D VolkowK Piscani
Feb 24, 1999·Alcoholism, Clinical and Experimental Research·L Korbo
Aug 22, 2001·Lancet·A CagninR B Banati
Aug 15, 2002·Pharmacology, Biochemistry, and Behavior·Jennifer A ObernierFulton T Crews
Jun 7, 2003·Proceedings of the National Academy of Sciences of the United States of America·Daniel G HerreraJ Manuel Garcia-Verdugo
Jun 7, 2007·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·George R BreeseRobert A Angel
Jan 15, 2008·Experimental Neurology·Jun He, Fulton T Crews
Apr 1, 2008·Pharmacology & Therapeutics·Ming-Kai Chen, Tomás R Guilarte
Nov 4, 2008·The British Journal of Psychiatry : the Journal of Mental Science·Susham Gupta, James Warner
Apr 29, 2010·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·David R OwenChristine A Parker
Jun 18, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Silvia Alfonso-LoechesConsuelo Guerri
Sep 17, 2011·Alcoholism, Clinical and Experimental Research·Catherine B FortierRegina E McGlinchey
Oct 20, 2011·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·David R OwenJustin P Rubio
Jun 19, 2012·Alcohol·Maciej KoperaWaldemar Szelenberger
Aug 10, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Sonia LavisseCarole Escartin
Jan 15, 2013·Neuron·Helmut KettenmannAlexei Verkhratsky
Sep 24, 2013·Biochimica Et Biophysica Acta·S V DemyanenkoL P Polyakova
Dec 3, 2013·Alcohol·Leandro F Vendruscolo, Amanda J Roberts
Feb 12, 2014·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Alex M DickensLaura Airas
Feb 26, 2014·Journal of Neurovirology·Jennifer M CoughlinMartin G Pomper
Apr 3, 2014·Alcoholism, Clinical and Experimental Research·John C UmhauMarkus Heilig
May 21, 2014·Progress in Neuro-psychopharmacology & Biological Psychiatry·Chelsea R GeilKimberly Nixon
Jan 20, 2015·Molecular Neurobiology·Yu Tang, Weidong Le
Feb 24, 2015·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Nora D VolkowDardo Tomasi
Mar 23, 2015·Biochemical and Biophysical Research Communications·Kristoffer SahlholmRobert H Mach

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