Detecting Receptor Tyrosine Kinase ROR1 Using a Developed Anti-ROR1 Polyclonal Antibody

Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
Azam HemmatiHodjattallah Rabbani

Abstract

Receptor tyrosine kinase ROR1 has been introduced as an interesting prognostic cancer marker in histopathology. The aim of this study was to produce a polyclonal antibody (PAb) against recombinant human ROR1 protein to be used as a tool for investigation of ROR1 expression in human cancer tissue blocks. The extracellular part of human ROR1 recombinant protein was expressed using pET-28b(+) plasmid in Escherichia coli Bl21(DE3) host. The recombinant ROR1, as a candidate immunogen, was purified and injected to a New Zealand rabbit. Followed by raising the titration of antibody, polyclonal anti-ROR1 antibody was purified through affinity chromatography column. After determining the purity of PAb anti-ROR1, its specific reactivity was assessed through various assessments. Flow cytometry analysis showed that PAb anti-ROR1 specifically recognizes ROR1 molecule in a number of positive and negative cell lines. Results obtained from detection of ROR1 in paraffin-embedded breast adenocarcinoma tissue blocks (n = 11) also demonstrated that PAb anti-ROR1 can effectively be used in immunohistochemistry. In conclusion, the developed anti-ROR1 PAb can be used as a tool for determining the prognostic value of ROR1 in histopathology of cancer t...Continue Reading

References

Apr 20, 2004·Critical Reviews in Oncology/hematology·Amar BennasrounePierre Hubert
Jan 29, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Sivasubramanian BaskarChristoph Rader
Jun 12, 2008·International Journal of Cancer. Journal International Du Cancer·Amir H DaneshmaneshHodjattallah Rabbani
Oct 14, 2008·Trends in Cell Biology·Jennifer L GreenPaul W Sternberg
Oct 9, 2012·The American Journal of Pathology·Suping ZhangThomas J Kipps
Nov 20, 2014·British Journal of Haematology·Amir Hossein DaneshmaneshHåkan Mellstedt
Aug 8, 2015·Annals of Diagnostic Pathology·Hyeyoon ChangBaek-hui Kim
Aug 25, 2015·Clinical Lymphoma, Myeloma & Leukemia·Michael Y ChoiThomas J Kipps
Jun 3, 2016·CA: a Cancer Journal for Clinicians·Kimberly D MillerAhmedin Jemal
Nov 11, 2016·Scientific Reports·Yu-Zhu ZhengYong Peng

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Citations

Apr 3, 2019·Expert Opinion on Therapeutic Targets·Amin KamraniMehdi Yousefi
Apr 23, 2019·Monoclonal Antibodies in Immunodiagnosis and Immunotherapy·Sajedeh BagheriMahdi Behdani
Aug 29, 2019·Iranian Journal of Biotechnology·Lia FarahiMohammad Mehdi Akhondi

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Methods Mentioned

BETA
PCR
electrophoresis
enzyme-linked immunosorbent assay
transfection
flow cytometry
ELISA

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