Detection and activity of peroxidase in the in situ formed enamel pellicle

Archives of Oral Biology
Christian HannigMatthias Hannig

Abstract

Peroxidase is the main salivary antioxidant. The aim of the present study was to detect and to characterise peroxidase in the in situ enamel pellicle. Bovine enamel slabs were fixed on maxillary splints and carried by six subjects for different times (3, 30 and 120 min) on buccal and palatal sites. Pellicle bound peroxidase activity was determined fluorimetrically using 2',7'-dichlorofluorescin as a substrate. The peroxidase molecules present in the pellicle were visualised with the gold-immunolabelling technique and evaluated by TEM. Furthermore, effects of polyphenols and hydrogen peroxide on peroxidase and its enzymatic activity were examined. All pellicles which were tested revealed peroxidase activity and labelled peroxidase molecules were detected in all samples. The numbers of gold-labelled peroxidase molecules detectable in cross-sections of the pellicles were correlated significantly with the pellicle formation time. After 3 min, 0.50+/-1.01 labelled molecules were detected (30 min: 1.42+/-1.98; 120 min: 4.15+/-4.13, ANOVA, p<0.001). The mean immobilised peroxidase activity exposed at the surface amounted to 24.4+/-27.7 mU/cm2; no continuous increase of activity with formation time was found. Hydrogen peroxide and poly...Continue Reading

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Citations

Sep 24, 2008·Clinical Oral Investigations·Christian HannigMatthias Hannig
Aug 11, 2010·Clinical Oral Investigations·Christian HannigThomas Attin
Mar 27, 2009·Archives of Oral Biology·C HannigM Hannig
Sep 1, 2015·European Journal of Oral Sciences·Susann GrychtolChristian Hannig
Mar 11, 2015·Clinical Oral Investigations·Elżbieta PaszynskaChristian Hannig
Jun 5, 2015·Archives of Oral Biology·Carolin BoehlkeChristian Hannig
Sep 2, 2016·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Carolin BoehlkeOliver Zierau
Jun 14, 2018·Caries Research·Viivi Karoliina AlaraudanjokiVuokko Anna-Marketta Anttonen
Jul 11, 2020·Bioengineering·Naji KharoufVincent Ball
Apr 1, 2021·European Journal of Oral Sciences·Natália Caldeira SilvaJonas Almeida Rodrigues
Jun 3, 2021·International Journal of Molecular Sciences·Jasmin FlemmingMatthias Hannig
Aug 3, 2021·International Journal of Dentistry·Amela TulekAmer Sehic
Jul 9, 2021·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Carolin BoehlkeChristian Hannig

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