PMID: 18638889Feb 1, 1983

Detection and cell-free translation of subgenomic RNAs of tobacco etch virus

Virology
T Otal, V Hari

Abstract

RNA extracted from purified tobacco etch virus and virus-infected tissue was examined by the northern hybridization technique and found to contain several subgenomic-sized viral RNA species in addition to the 3 x 10(6) MW genomic RNA. Of the 10 subgenomic-sized RNAs detected (2.03, 1.24, 1.14, 1.01, 0.84, 0.62, 0.55, 0.46, 0.34, and 0.28 x 10(6) MW), 4 were found in both virion RNA and infected tissue RNA, 3 others only in virion RNA, and 3 only in infected tissue RNA. Of the last three, two may be engendered during isolation by nuclease action on virion RNA, as indicated by controls. On the basis of their binding to oligo(dT) cellulose, all of the RNAs found in virus-infected tissue appeared to be polyadenylated. Cell-free translation of electrophoretically purified genome-length TEV RNA in a rabbit reticulocyte system stimulated the synthesis of a 39,000 MW polypeptide. In contrast, translation of unfractionated virion RNA in the reticulocyte system stimulated the production of a number of polypeptides; three of the principal products had molecular weights corresponding closely to those of known virus-encoded proteins. The predominant cell-free translation product of both electrophoretically purified genomic RNA and unfractio...Continue Reading

References

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Related Concepts

Genome
Reticulocytes
RNA
RNA, Viral
Transcription, Genetic
Virion
Virus Diseases
Virus
Wheat (Dietary)
oligo(dT)-cellulose

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