Detection and characterization of a clinical Escherichia coli ST3204 strain coproducing NDM-16 and MCR-1

Infection and Drug Resistance
Xi LiYunsong Yu

Abstract

A plasmid-mediated colistin resistance gene, mcr-1, has been reported worldwide and has caused concern regarding a major therapeutic challenge. Alarmingly, mcr-1 has spread into clinical carbapenem-resistant Enterobacteriaceae isolates, resulting in extensively drug-resistant and even pan drug-resistant isolates that can cause untreatable infections. In this study, we report isolation of an extensively drug-resistant Escherichia coli strain EC1188 that coproduces NDM-16 and MCR-1 from a urine sample taken from a patient with craniocerebral injury. E. coli strain EC1188 was identified and subjected to genotyping, susceptibility testing and conjugation experiments. The genetic locations of blaNDM-16 and mcr-1 were established with southern blot hybridization. The complete genome sequence of this strain was obtained and the genetic characteristics of the mcr-1- and blaNDM-16-harboring plasmids were analyzed. In addition, comparative genetic analyses of mcr-1 and blaNDM-16 with closely related plasmids were also carried out. Whole-genome sequencing revealed that strain EC1188 possess various resistance genes and virulence genes. S1-pulsed-field gel electrophoresis and southern blot suggested that the blaNDM-16 and mcr-1 genes were ...Continue Reading

Citations

Apr 5, 2019·PloS One·Jocelin Merida-VieyraAlejandra Aquino-Andrade
Sep 5, 2019·Frontiers in Microbiology·Xiaoyan CuiHong Du
Jan 7, 2021·Future Microbiology·Muhammad Usman QamarMark A Toleman

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Datasets Mentioned

BETA
MF353156
CP026201

Methods Mentioned

BETA
electrophoresis

Software Mentioned

MLST
Plasmid Finder
Virulence Finder
BLASTn
ResFinder
EasyFig

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