Detection and characterization of B cell epitopes on beta2-glycoprotein I

Clinical Immunology : the Official Journal of the Clinical Immunology Society
Keith A CockerillG Michael Iverson

Abstract

Autoantibodies in antiphospholipid syndrome react predominantly with the plasma protein beta2-glycoprotein I (beta2GPI). Work by a large number of investigators has led to considerable progress in detecting and understanding beta2GPI reactivity with autoantibodies. Characterization of B cell epitopes on beta2GPI has benefited from an appreciation of its interactions with anionic phospholipids and a variety of microplate surfaces. In particular, autoantibodies to beta2GPI are of sufficiently low affinity to require high concentrations of antigen for detectable reactivity. Moreover, some microplate surfaces do not support the proper orientation of beta2GPI to allow display of epitopes in a manner accessible to autoantibodies. These concepts have helped to explain previous notions that exposure of cryptic beta2GPI epitopes may require interactions with anionic surfaces. Finally, we review evidence identifying a dominant B cell epitope that is partially defined by residues Gly40 and Arg43 on the amino terminal domain of beta2GPI.

References

Oct 25, 1991·Journal of Immunological Methods·J ArvieuxM G Colomb
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·H P McNeilS A Krilis
Mar 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·J E HuntS A Krilis

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Citations

Nov 22, 2005·Clinical and Experimental Immunology·S OwensP M Johnson
Jul 9, 2004·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Robert A S Roubey
Aug 30, 2005·Annals of the New York Academy of Sciences·Guido Valesini, Cristiano Alessandri

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Related Concepts

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Antiphospholipid Syndrome

Antiphospholipid syndrome or antiphospholipid antibody syndrome (APS or APLS), is an autoimmune, hypercoagulable state caused by the presence of antibodies directed against phospholipids.

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