Detection and characterization of circulating microsatellite-DNA in blood of patients with breast cancer

Annals of the New York Academy of Sciences
Heidi SchwarzenbachKlaus Pantel

Abstract

Increased levels of circulating DNA have been reported in the blood of cancer patients but not healthy individuals. Tumor-specific genomic aberrations, such as loss of heterozygosity (LOH) and microsatellite instability (MSI), can be detected in this free extracellular DNA. Identification of these genetic aberrations may play an important role in cancer diagnosis and prediction of disease progression. Moreover, the genomic regions involved might harbor potential targets for therapies. To evaluate the incidence of microsatellite alterations in circulating DNA, we assessed the blood serum of 34 patients with primary (n = 8) and metastatic (n = 24) breast cancer. Samples were also analyzed for the presence of circulating tumor cells using an immunocytological cytokeratin assay, and the concentration of the tumor marker CA 15-3 was determined. Genomic DNA extracted from serum and normal blood leukocytes, as a control, was amplified by the polymerase chain reaction using markers at 4 microsatellite loci of chromosomes 10q22-23, 16q22-23, 17q11-12, and 17q21. In 17 of 34 cancer patients, tumor-specific alterations were detected in serum samples. In 16 patients, LOH at various loci was observed, whereas MSI was only detected in the se...Continue Reading

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Citations

Oct 31, 2012·Cancer Metastasis Reviews·David S GutteryJacqueline A Shaw
Jul 26, 2011·Molecular BioSystems·Heidi SchwarzenbachKlaus Pantel
Aug 28, 2007·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·Robin WachowiakJakob R Izbicki
Nov 5, 2010·Breast Cancer Research : BCR·Carina RothHeidi Schwarzenbach
Nov 19, 2010·Bioanalysis·Noha Gerges, Nada Jabado
Aug 7, 2010·Clinica Chimica Acta; International Journal of Clinical Chemistry·Klaus JungAnja Rabien
Sep 15, 2009·Clinical Biochemistry·Maniesh van der Vaart, Pieter J Pretorius
Jun 24, 2008·The Breast : Official Journal of the European Society of Mastology·Julian E LosanoffEdward R Sauter
May 12, 2007·Trends in Biotechnology·Chunming Ding
Oct 8, 2008·Annals of the New York Academy of Sciences·Heidi SchwarzenbachEray Goekkurt
Sep 1, 2007·Veterinary and Comparative Oncology·D M W SchaeferL J Rush
Apr 25, 2007·Pathology·Jason C H Tsang, Y M Dennis Lo
Dec 2, 2006·Biochimica Et Biophysica Acta·M Fleischhacker, B Schmidt
Mar 18, 2009·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Friedemann HoneckerLeendert H J Looijenga
Dec 16, 2017·Cancer Investigation·Shahram AgahFarinaz Nazmi
Mar 14, 2008·BJU International·Heidi SchwarzenbachMartin G Friedrich
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Oct 20, 2006·Critical Reviews in Clinical Laboratory Sciences·Qinghua FengNancy B Kiviat
Jan 6, 2007·International Journal of Cancer. Journal International Du Cancer·Heidi SchwarzenbachKlaus Pantel
Dec 11, 2007·Expert Review of Proteomics·Karine JacobNada Jabado
Jan 27, 2017·Biomedical Reports·Shankar SurajSweta Srivastava
Feb 4, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Heidi SchwarzenbachKlaus Pantel

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