Detection and characterization of hemopoietic stem cells in the adult human small intestine

The Journal of Immunology : Official Journal of the American Association of Immunologists
Lydia LynchL Golden-Mason

Abstract

The concept of lymphoid differentiation in the human gastrointestinal tract is controversial but is the focus of this study, which examined adult human small intestinal tissue for the presence of CD34(+)CD45(+) hemopoietic stem cells (HSCs) and lymphoid progenitors. Flow cytometry demonstrated that over 5% of leukocytes (CD45(+) cells) isolated from human gut were HSCs coexpressing CD34, a significantly higher incidence than in matched peripheral blood or control bone marrow. HSCs were detected in cell preparations from both the epithelium and lamina propria of all samples tested and localized to the intestinal villous and crypt regions using immunofluorescence. A high proportion of gut HSCs expressed the activation marker CD45RA, and few expressed c-kit, indicating ongoing differentiation. The vast majority of intestinal HSCs coexpressed the T cell Ag, CD7 (92% in the epithelium, 80% in the lamina propria) whereas <10% coexpressed the myeloid Ag CD33, suggesting that gut HSCs are a relatively mature population committed to the lymphoid lineage. Interestingly, almost 50% of epithelial layer HSCs coexpressed CD56, the NK cell Ag, compared with only 10% of the lamina propria HSC population, suggesting that the epithelium may be a...Continue Reading

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Citations

Jan 31, 2018·American Journal of Reproductive Immunology : AJRI·Louise E GloverCliona O'Farrelly
Sep 20, 2006·BMC Infectious Diseases·Thomas TsaganosHelen Giamarellou
Jul 27, 2012·International Journal of Gynecological Pathology : Official Journal of the International Society of Gynecological Pathologists·Anne M MillsTeri A Longacre
Sep 24, 2014·Journal of Leukocyte Biology·Tatyana Lysakova-Devine, Cliona O'Farrelly
Jul 25, 2008·Blood·Michael A Caligiuri
Aug 24, 2010·HIV Therapy·Pavel BostikAftab A Ansari
Dec 7, 2013·Immunology·Binqing FuHaiming Wei
May 20, 2015·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·J ZuberM Sykes
Sep 7, 2020·Developmental and Comparative Immunology·Nathalie MeijerinkChristine A Jansen
Aug 12, 2009·The Journal of Surgical Research·Frédéric G SalaTracy C Grikscheit
Oct 24, 2021·Experimental Hematology·Nicole Mende, Elisa Laurenti

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