Jan 1, 1987

Detection and characterization of lipid hydroperoxides at picomole levels by high-performance liquid chromatography

Analytical Biochemistry
Y YamamotoB N Ames

Abstract

A new method for the detection of various lipid hydroperoxides and hydrogen peroxide at the picomole level has been developed by combining an HPLC system with an ultrasensitive analytical system based on the detection of chemiluminescence emitted by isoluminol in the presence of hydroperoxide and microperoxidase. This HPLC separation removes interfering antioxidants so that the method can be applied to biological samples such as blood plasma lipids. Several HPLC conditions are described which allow simple identification of different lipid hydroperoxides.

  • References1
  • Citations154

References

Mentioned in this Paper

Ovoperoxidase
Hydrogen Peroxide
Nickel microperoxidase-11
Antioxidant Effect
Antioxidants
Lipid Peroxides
High Pressure Liquid Chromatography Procedure
Lipids
Oxydol
Isoluminol

About this Paper

Related Feeds

CZI Human Cell Atlas Seed Network

The aim of the Human Cell Atlas (HCA) is to build reference maps of all human cells in order to enhance our understanding of health and disease. The Seed Networks for the HCA project aims to bring together collaborators with different areas of expertise in order to facilitate the development of the HCA. Find the latest research from members of the HCA Seed Networks here.