Detection and characterization of metallo beta lactamases producing Pseudomonas aeruginosa

Indian Journal of Medical Microbiology
A ManoharanSARI Study Group

Abstract

This study was undertaken to evaluate phenotypic and genotypic methods for detection of Metallo-Beta-Lactamases (MBLs) among nosocomial Pseudomonas aeruginosa. Sixty one among 176 P. aeruginosa isolates, collected as part of a multicentric study (2005-2007), were evaluated for carbapenem resistance (CARB-R; resistant to either imipenem/meropenem) and screened for MBL by Combination Disk Diffusion Test (CDDT) using imipenem (IMP), meropenem (MER) and ceftazidime (CAZ) with EDTA. MBL positives were further confirmed by IMP + EDTA Etest. Twenty strains (42.6%) were found to be MBL producers among the 61 P. aeruginosa. PCR for IMP and VIM MBL was performed on 48 of the 61, 15 were positive for VIM MBL type. CDDT using IMP + EDTA had the highest sensitivity and specificity of 87.8% and 84.4% when compared to Etest, which was higher than the values obtained for CAZ + EDTA and MER + EDTA. CDDT using IMP + EDTA also compared very well with the PCR (specificity = 90.9%, sensitivity = 93.3%). CARB-R among P. aeruginosa is mediated predominantly via MBL production. Clinical P. aeruginosa isolates can be screened routinely using the less expensive IMP + EDTA CDDT in clinical microbiology laboratories.

References

Apr 16, 2005·Clinical Microbiology Reviews·Timothy R WalshPatrice Nordmann
Jul 8, 2005·Journal of Clinical Microbiology·Johann D D PitoutDeirdre L Church
Apr 4, 2007·Antimicrobial Agents and Chemotherapy·Oscar CirioniAndrea Giacometti

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Citations

Jul 24, 2014·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Beena Benita D'SouzaPriyanka Pothen
Mar 15, 2015·Infectious Diseases·Ramanathan ThamizhmaniPaluru Vijayachari
Feb 24, 2015·Current Medical Research and Opinion·Abed Zahedi Bialvaei, Hossein Samadi Kafil
Apr 7, 2016·Indian Journal of Medical and Paediatric Oncology : Official Journal of Indian Society of Medical & Paediatric Oncology·Vivek BhatTejpal Gupta
Jan 3, 2013·Microbiology and Immunology·Piyatip KhuntayapornMullika Traidej Chomnawang
Dec 31, 2016·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Iara Rossi GonçalvesRosineide Marques Ribas

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