Detection and characterization of porcine endogenous retrovirus in porcine plasma and porcine factor VIII

Journal of Virology
Daniel M TakefmanC A Wilson

Abstract

The pig genome contains porcine endogenous retroviruses (PERVs) capable of infecting human cells. Detection of infectious retrovirus in porcine peripheral blood mononuclear cells and endothelial cells suggested to us that pig plasma is likely to contain PERV. Both PERV env sequences and viral reverse transcriptase (RT) activity were detected in all plasma samples isolated from four NIH minipigs. To detect infectious virus from plasma, we performed a culture assay using three cell lines of feline, swine, and human origin that had previously been shown to be permissive for PERV. Infectious virus was successfully cultured from all four NIH minipig plasmas on the swine cell line ST-IOWA. Using RT-PCR with env-specific primers, we could detect expression of PERV class C envelope in the supernatant of ST-IOWA cells that had been exposed to each pig plasma. We next examined a pig plasma derivative, Hyate:C (porcine factor VIII), and found evidence of PERV particles, since all six lots examined were positive for PERV RNA and RT activity. However, infectious virus could not be detected in clinical lots of Hyate:C, suggesting that the manufacturing process might reduce the load of infectious virus to levels below detectable limits of the...Continue Reading

References

Aug 1, 1975·Virology·M M LieberG J Todaro
Oct 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·R E Benveniste, G J Todaro
Feb 1, 1971·The Journal of General Virology·J A ArmstrongA T De Madrid
Mar 1, 1997·Nature Medicine·C PatienceR A Weiss
Oct 24, 1997·Nature·P Le TissierR A Weiss
Mar 12, 1998·Infectious Disease Clinics of North America·M Chamberland, R F Khabbaz
Apr 29, 1998·Journal of Virology·D E AkiyoshiJ A Fishman
Nov 13, 1998·Journal of Virology·Y TakeuchiJ P Stoye
Dec 10, 1999·Journal of Virology·C A WilsonM J Federspiel

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Citations

Oct 9, 2002·Journal of Virological Methods·Takele ArgawCarolyn A Wilson
Mar 28, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Christopher B DoeringH Trent Spencer
Apr 12, 2012·Clinical Microbiology Reviews·Joachim Denner, Ralf R Tönjes
Sep 27, 2003·Journal of Virology·Nikolai KlymiukBernhard Aigner
Jun 13, 2002·Xenotransplantation·Jürgen H BluschUlrich Martin
Jun 17, 2008·Transplantation Proceedings·E DörrschuckR R Tönjes
Jun 11, 2003·Xenotransplantation·Maritza C McIntyreMatiza C McIntyre
Jul 22, 2006·Journal of Thrombosis and Haemostasis : JTH·R T Barrow, P Lollar
Jan 13, 2009·Veterinary Surgery : VS·Sandra P SchallbergerBarbara A Steficek
Apr 26, 2012·Haemophilia : the Official Journal of the World Federation of Hemophilia·P L F Giangrande
Feb 7, 2015·Haemophilia : the Official Journal of the World Federation of Hemophilia·P M Mannucci
Aug 19, 2015·Haemophilia : the Official Journal of the World Federation of Hemophilia·D LillicrapP Lollar
Mar 31, 2016·Xenotransplantation·Takele ArgawCarolyn Wilson
Aug 22, 2003·Reviews in Medical Virology·Saema MagreBirke Bartosch
Nov 8, 2007·Reviews in Medical Virology·Derrick LouzRob C Hoeben
May 3, 2005·Transplant International : Official Journal of the European Society for Organ Transplantation·Monica E WinklerUlrich Martin

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