PMID: 698195Sep 5, 1978Paper

Detection and characterization of the intermediate on the folding pathway of human alpha-lactalbumin

Biochemistry
M NozakaS Sugai

Abstract

To discuss the relation between the folding mechanism and the chemical structure of proteins, the reversible unfolding reactions of human alpha-lactalbumin by acidification and by guanidine hydrochloride at 25 degrees C are studied by means of circular dichroism, difference spectra and pH-jump measurements and are compared with those for bovine alpha-lactalbumin. As shown previously for bovine alpha-lactalbumin, the folding process at neutral pH is not explained by a simple two-state mechanism but involves an intermediate form that has the same amount of helical structures as the native form. The transition between the intermediate and the fully denatured states is too rapid to be measured and corresponds to the helix-coil transition of the backbone. One of the differences of human alpha-lactalbumin from the bovine protein is the remarkable stability of the intermediate at neutral pH, which can be explained by differences in the primary chemical structure. Another difference is the existence at acid pH of an additional helical form, which is more helical than the native form. The transition from this to the intermediate or to the fully denatured one also is shown to resemble the helix-coil transition. The following folding sche...Continue Reading

References

Sep 15, 1976·Journal of Molecular Biology·K KuwajimaS Sugai
Mar 1, 1977·Macromolecules·S Tanaka, H A Scheraga
Jan 1, 1975·Advances in Protein Chemistry·C B Anfinsen, H A Scheraga
Apr 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·S Tanaka, H A Scheraga
Nov 5, 1976·Journal of Molecular Biology·B Robson, E Suzuki
Oct 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·S Tanaka, H A Scheraga
Jan 1, 1975·Annual Review of Biochemistry·R L Baldwin
Feb 1, 1975·Biophysical Chemistry·O B Ptitsyn, A A Rashin
Jan 15, 1974·Biochemistry·P Y Chou, G D Fasman
Jan 1, 1972·Methods in Enzymology·Y Nozaki
Jan 1, 1968·Advances in Protein Chemistry·C Tanford
May 1, 1972·European Journal of Biochemistry·J B Findlay, K Brew
Jan 1, 1972·Biopolymers·K Nitta, S Sugai

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Citations

Jul 31, 1998·Protein Science : a Publication of the Protein Society·M IkeguchiS Sugai
Oct 29, 1998·Protein Science : a Publication of the Protein Society·M KikuchiK Nitta
Nov 1, 1995·Journal of Protein Chemistry·S GohdaS Sugai
Mar 7, 1996·Biochimica Et Biophysica Acta·J M AraminiH J Vogel
Feb 5, 2002·Biochimica Et Biophysica Acta·Konstantin S Vassilenko, Vladimir N Uversky
Aug 1, 1992·The Journal of Dairy Research·R L Lyster
Oct 24, 2002·European Journal of Biochemistry·Gyu Hyun Nam, Kwan Yong Choi
Nov 26, 2009·The Journal of Biological Chemistry·Ana Paula D Ano BomJerson L Silva
Jan 13, 2000·Protein Science : a Publication of the Protein Society·Y KobashigawaK Nitta
May 26, 2012·Biological Chemistry·Sergei E PermyakovEugene A Permyakov
Jul 1, 1992·Biological Chemistry Hoppe-Seyler·E ZerovnikR H Pain
Feb 21, 2016·Computational Biology and Chemistry·Aleksei KuznetsovJaak Järv
Dec 28, 1981·FEBS Letters·D A DolgikhO B Ptitsyn
May 21, 2011·Biochimie·Sergei E PermyakovEugene A Permyakov
Mar 25, 2008·Biochimica Et Biophysica Acta·Dmitry A ProkhorovViktor P Kutyshenko
Jun 5, 2003·Proteins·Eugene A PermyakovVladimir N Uversky
Aug 14, 1980·Biochemical and Biophysical Research Communications·Y HiraokaN Murai
Nov 10, 2004·Journal of Molecular Biology·Bryan A KrantzR John Collier
Oct 29, 2003·Biophysical Journal·Paulo Roberto LouzadaSérgio T Ferreira
Sep 26, 2002·Journal of Biochemistry and Molecular Biology·Monica SunddMedicherla V Jagannadham
Aug 12, 2014·Biophysical Chemistry·Izlia J Arroyo-MayaAndrés Hernández-Arana
Jun 18, 1991·Biochemistry·D O Alonso, K A Dill
Apr 20, 1993·Biochemistry·L R De YoungA L Fink

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