PMID: 15253500Jul 16, 2004Paper

Detection and enumeration of Cryptosporidium sp. oocysts in sewage sludge samples from the city of Florianópolis (Brazil) by using immunomagnetic separation combined with indirect immunofluorescence assay

Waste Management & Research : the Journal of the International Solid Wastes and Public Cleansing Association, ISWA
Roniele Balvedi IacovskiCláudia Simões

Abstract

In the last few decades, agricultural reclamation of sewage sludge has increased in many countries. However, this practice must be adopted under recommended conditions to limit the risks associated with the presence of potential pathogens, including protozoan parasites such as Cryptosporidium. In the present work, we evaluated immunomagnetic separation (IMS) combined with an indirect immunofluorescence assay (IFA) in order to detect Cryptosporidium oocysts in sewage sludge samples obtained from the Municipal Wastewater Treatment Plant (MWTP) operating in the city of Florianópolis, State of Santa Catarina, Brazil. Integrated application of spontaneous sludge sedimentation and centrifugation of the supernatant, purification and concentration of oocysts through immunomagnetic separation as well as detection and quantification by means of indirect immunofluorescence using highly specific monoclonal antibodies, proved to be an effective multiple-analysis tool for monitoring the presence of Cryptosporidium oocysts in urban sewage sludge samples. Among 27 samples collected from MWTP, 20 (74.07%) were positive for Cryptosporidium sp. The efficiency of recovery by using the internal control ColorSeed (Biotechnology Frontiers Inc., Sydne...Continue Reading

References

May 8, 2001·Revista Do Instituto De Medicina Tropical De São Paulo·R M FrancoR Cantusio Neto
Feb 20, 2002·Journal of Applied Microbiology·R Rimhanen-FinneM L Hänninen

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Citations

Dec 11, 2008·Zoonoses and Public Health·L NishiA L Falavigna-Guilherme
Aug 24, 2007·Tropical Medicine & International Health : TM & IH·M C A TeixeiraN M Alcântara-Neves
Apr 16, 2016·Environmental Technology·Inmaculada AmorósJose L Alonso
Dec 28, 2010·Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária·Marcelo Vasconcelos Meireles
May 29, 2019·Environmental Technology·Gabriela Laila de Oliveira, Luiz Antonio Daniel
Jan 19, 2021·Water Science and Technology : a Journal of the International Association on Water Pollution Research·Eman M HassanAsma Iqbal

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