Detection and genetic characterisation of Hepatitis E virus in Czech pig production herds

Research in Veterinary Science
P VasickovaP Kralik

Abstract

The objective of the present study was to carry out a small surveillance programme in Czech pig production herds using the nested reverse transcriptase-polymerase chain reaction (nRT-PCR) technique to trace Hepatitis E virus (HEV) in different biological samples and to characterise the detected swine HEV isolates by phylogenetic analysis. A total of 32 piglets from 11 herds clinically suspected of postweaning multisystemic wasting syndrome (PMWS) were examined. Bile, liver tissue and serum samples were collected from each animal. Due to the high genetic variability of HEV, three sets of primers targeting each of the open reading frames (ORFs) of its genome were used. HEV RNA was most frequently detected in the bile samples (40.0%), followed by liver tissue (16.1%) and serum (3.2%). Seven (63.6%) of the 11 monitored farms were found to have at least one HEV RNA positive piglet. Specific 242 bp sequences within the ORF1 coding non-structural proteins were sequenced and phylogenetically analysed. Phylogenetic analysis using the neighbor-joining and maximum parsimony method confirmed that all detected Czech swine HEV isolates belonged to genotype III. Comparison of the Czech swine HEV isolates with corresponding sequences of swHEV ...Continue Reading

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Citations

Nov 28, 2013·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Pavel ChalupaMichal Holub
May 3, 2011·Emerging Infectious Diseases·Petra VasickovaIvo Pavlik
Jan 23, 2010·Research in Veterinary Science·F MartelliF Ostanello
Mar 9, 2010·International Journal of Food Microbiology·Danielle LeblancAlain Houde
Jul 5, 2015·Food and Environmental Virology·Anna IvanovaIrina Golovljova
Mar 17, 2015·Food and Environmental Virology·Monika KubankovaPetra Vasickova
Aug 2, 2011·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Andrej SteyerMario Poljak
Jul 18, 2018·Transboundary and Emerging Diseases·Petra StrakovaZdenek Hubalek
Feb 24, 2018·Acta Veterinaria Scandinavica·Ugne SpancernieneArunas Stankevicius
Jul 31, 2012·Emerging Infectious Diseases·Ilaria Di BartoloFranco Maria Ruggeri
Jul 2, 2021·BMC Veterinary Research·Anna JackovaStefan Vilcek

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