Detection and haplotype differentiation of Southeast Asian alpha-thalassemia using polymerase chain reaction and a piezoelectric biosensor immobilized with a single oligonucleotide probe

Translational Research : the Journal of Laboratory and Clinical Medicine
Phantip VattanaviboonChamras Promptmas

Abstract

DNA-based diagnosis of alpha-thalassemias routinely relies on polymerase chain reaction (PCR) and gel electrophoresis. Here, we developed a new procedure for the detection and haplotype differentiation of Southeast Asian (SEA) alpha-thalassemia using a 3-primer system for PCR coupling with a DNA-based piezoelectric biosensor. PCR products amplified from genomic DNA were differentiated directly by using a quartz crystal microbalance immobilized with a single oligonucleotide probe. The frequency changes after hybridization of the PCR products amplified from a representative sample of normal alpha-globin, SEA alpha-thalassemia heterozygote, and homozygote were 206+/-11, 256+/-5, and 307+/-3 Hz, respectively. The fabricated biosensor was evaluated through an examination of 18 blind specimens. It could accurately discriminate between normal and SEA alpha-thalassemic samples, which suggests that this biosensor system is a promising alternative technique to detect SEA alpha-thalassemia because of its specificity and less hazardous exposure as compared with conventional methods.

References

Mar 29, 2000·Annals of Clinical Biochemistry·J E PearsonP Vadgama
Oct 22, 2003·Biosensors & Bioelectronics·Marin Gheorghe, Anthony Guiseppi-Elie
Oct 12, 2005·Methods : a Companion to Methods in Enzymology·Sara TombelliMarco Mascini

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Citations

Feb 9, 2013·Clinica Chimica Acta; International Journal of Clinical Chemistry·Wanida IttaratWarunee Ngrenngarmlert
Jun 29, 2010·Clinical Chemistry and Laboratory Medicine : CCLM·Sirinart ChomeanWanida Ittarat
Dec 2, 2011·Journal of Biomedicine & Biotechnology·D Dey, T Goswami
Aug 4, 2011·Journal of Molecular Recognition : JMR·Bernd Becker, Matthew A Cooper
Feb 9, 2013·Clinical Chemistry and Laboratory Medicine : CCLM·Nantawan WangmaungWanida Ittarat

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