Detection and identification of antibacterial proteins in snake venoms using at-line nanofractionation coupled to LC-MS

Toxicon : Official Journal of the International Society on Toxinology
Marija MladicMichael K Richardson

Abstract

This study describes the application of at-line nanofractionation to the screening of snake venoms for antibacterial activity against Gram-positive and Gram-negative bacteria, the detection of proteins of interest, and their partial or full identification. A method was developed to identify bioactive peptides in crude snake venoms based on reversed-phase liquid chromatography (LC), with parallel nanofractionation onto 384-well plates and mass spectrometry (MS). Bioactivity assays were based on a resazurin-reduction assay. Accurate masses of the bioactive peptides were determined, and peptides were then identified via nanoLC-MS/MS analysis of tryptic digests, allowing full or partial identification of the bioactive proteins. Crude venoms from 41 species were screened for their antibacterial bioactivity. Venoms showing the highest activity were further screened using at-line nanofractionation, which resulted in the elucidation of 28 bioactive proteins.

References

Dec 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·M Sekiguchi, S Iida
Jan 22, 1968·Biochimica Et Biophysica Acta·S Aloof-HirschA Berger
Nov 30, 1999·American Heart Journal·R M Scarborough
May 2, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Charles G Smith, John R Vane
May 21, 2005·Toxicon : Official Journal of the International Society on Toxinology·Carlos SantamaríaBruno Lomonte
Oct 19, 2006·The Biochemical Journal·Dileep G NairR Manjunatha Kini
May 20, 2009·Antimicrobial Agents and Chemotherapy·Zhenhuan ZhaoWenxin Li
Sep 24, 2011·Expert Opinion on Biological Therapy·Glenn F King
Nov 20, 2012·Toxicon : Official Journal of the International Society on Toxinology·Ferry HeusJeroen Kool
May 10, 2013·Cellular and Molecular Life Sciences : CMLS·Nelson Gomes de Oliveira JuniorOctavio Luiz Franco
Oct 22, 2013·Toxicon : Official Journal of the International Society on Toxinology·Reka A OtvosJeroen Kool
Dec 29, 2015·Toxicon : Official Journal of the International Society on Toxinology·Marija MladicJeroen Kool
Aug 13, 2017·Analytical and Bioanalytical Chemistry·Marija MladicJeroen Kool

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Citations

Aug 24, 2020·Toxicon : Official Journal of the International Society on Toxinology·Jia-Qi LiXiang Ji
Sep 29, 2021·Journal of Enzyme Inhibition and Medicinal Chemistry·Xiao-Wei ZhangDe-Qiang Li

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