Detection and identification of avian, duck, and goose reoviruses by RT-PCR: goose and duck reoviruses are part of the same genogroup in the genus Orthoreovirus

Archives of Virology
Y ZhangZ Han

Abstract

A reverse transcription-polymerase chain reaction (RT-PCR) procedure for the detection of avian, duck, and goose reovirus (ARV, DRV, and GRV) RNA from cell culture supernatant and clinical samples was established. Based on multiple sequence alignment, a pair of degenerate primers was selected and synthesized. The amplified, cloned, and sequenced 598-base-pair products from the sigmaA-encoding gene fragment from 16 isolates (ranging over 30 years) indicated that the primer regions were well conserved. The sensitivity of this method was determined to be 10(-2) PFU. The specificity of the RT-PCR method was determined by testing specimens containing avian influenza A viruses, Newcastle disease virus, and infectious bronchitis virus, all of which yielded negative results with no discernible background. The efficiency of the system for detection of ARV, DRV, and GRV directly in 71/83 clinical samples was confirmed. The nucleotide sequence analysis indicated that DRV and GRV isolated from China in different locales and years were closely related, showing 97.4-100% homology to each other, but with only 86.7-88.5% identity to DRV 89026. The nucleotide and amino acid sequence identities in the amplified sigmaA-encoding gene were 74.2-78....Continue Reading

References

Jul 2, 1988·The Veterinary Record·V Marius-JestinG Bennejean
Sep 1, 1987·Journal of Comparative Pathology·B M AdairE R McKillop
Dec 24, 1997·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·S L ThomasA J Hall
Feb 6, 1999·The Journal of General Virology·G Le Gall-ReculéV Jestin
Apr 20, 2000·American Journal of Veterinary Research·M FaberA Barneveld
Jun 17, 2000·Journal of Virological Methods·A P Wyn-JonesJ Sellwood
Sep 30, 2000·Journal of Virological Methods·O LegeayM Lange
Sep 29, 2001·Journal of Virological Methods·H J Liu, P H Huang
Apr 19, 2002·The Journal of General Virology·Gaëlle Kuntz-SimonVéronique Jestin
May 15, 2003·Avian Pathology : Journal of the W.V.P.A·Vilmos PalyaMária Benko
Dec 29, 2004·Journal of Virological Methods·Silke BruhnHans-Peter Ottiger
Apr 11, 2006·Virus Genes·Yun ZhangGuangzhi Tong
Sep 1, 1992·Avian Pathology : Journal of the W.V.P.A·U Heffels-RedmannE F Kaleta
Apr 1, 1984·Avian Pathology : Journal of the W.V.P.A·R C Jones, J R Guneratne
Oct 1, 1981·Avian Pathology : Journal of the W.V.P.A·M MalkinsonY Weisman

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Citations

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