Detection and identification of avian mycoplasmas by polymerase chain reaction and restriction fragment length polymorphism assay

Veterinary Microbiology
I KissK E Johansson

Abstract

The polymerase chain reaction (PCR) with primers complementary to the 16S rRNA genes was used to detect avian mycoplasmas. A primer pair designed for the detection of human and rodent mycoplasmal species was examined for its ability to detect the most important avian mycoplasmas. After testing the respective reference strains, we found that Mycoplasma iowae, Mycoplasma meleagridis and Mycoplasma synoviae could be detected by PCR with this primer pair, and distinction could be made among them by restriction fragment length polymorphism (RFLP) assay with two restriction enzymes (BamHI and RsaI). For the detection of Mycoplasma gallisepticum by PCR, we needed species-specific primers. The results of the PCR- and RFLP, based identification procedures of 17 different field isolates agreed with those obtained by conventional methods.

Citations

Sep 9, 2005·Avian Pathology : Journal of the W.V.P.A·D R Mekkes, A Feberwee
Dec 23, 2011·Journal of Clinical Microbiology·Pálma SillóSarolta Kárpáti
Jun 16, 2005·BMC Infectious Diseases·Tim StakenborgMario Vaneechoutte
May 7, 2011·Veterinary Medicine International·R L LucianoE N C Tessari
Apr 7, 2015·Journal of Avian Medicine and Surgery·Tom HellebuyckAn Martel
Aug 12, 2005·Experimental Animals·Dong-Jae KimJae-Hak Park
Jan 13, 2018·Journal of Avian Medicine and Surgery·Tom HellebuyckAn Martel

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