Jul 19, 2008

Detection and identification of bacteria intimately associated with fungi of the order Sebacinales

Cellular Microbiology
Monica SharmaKarl-Heinz Kogel

Abstract

Because of their beneficial impact on plants, the highly diverse mycorrhizal fungi grouped in the order Sebacinales lay claim to high ecological and agricultural significance. Here, we describe for the first time associations of Sebacinoid members with bacteria. Using quantitative PCR, denaturating gradient gel electrophoresis and fluorescence in situ hybridization, we detected an intimate association between Piriformospora indica and Rhizobium radiobacter, an alpha-Proteobacterium. The stability of the association, vertical transmission of the bacteria during asexual fungal reproduction and fungal plant colonization was monitored using R. radiobacter-specific primers. Treatment of mycelium or fungal protoplasts with antibiotics highly efficient against the free bacteria failed to cure the fungus. Barley seedlings dip-inoculated with R. radiobacter showed growth promotion and systemic resistance to the powdery mildew fungus Blumeria graminis comparable to P. indica inoculation. By screening additional isolates of the Sebacina vermifera complex, three species-specific associations with bacteria from the genera Paenibacillus, Acinetobacter and Rhodococcus were found. These findings suggest that Sebacinales species regularly under...Continue Reading

Mentioned in this Paper

Perotis indica
Fluorescent in Situ Hybridization
Host-Parasite Interactions
Sebacinales
Mutualism
Perna indica
Antibiotic throat preparations
Agrobacterium tumefaciens
Pyrenochaetopsis indica
Blumeria graminis

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