Detection and identification of methicillin resistant and sensitive strains of Staphylococcus aureus using tandem measurements

Journal of Microbiological Methods
Rajesh GuntupalliVitaly Vodyanoy

Abstract

Discrimination of methicillin resistant (MRSA) and sensitive (MSSA) strains of Staphylococcus aureus, was achieved by the specially selected lytic bacteriophage with a wide host range of S. aureus strains and a penicillin-binding protein (PBP 2a) specific antibody. A quartz crystal microbalance with dissipation monitoring (QCM-D) was employed to analyze bacteria-phage interactions. The lytic phages were transformed into phage spheroids by exposure to water-chloroform interface. Phage spheroid monolayers were transferred onto QCM-D sensors by Langmuir-Blodgett (LB) technique. Biosensors were tested in the flow mode with bacterial water suspensions, while collecting frequency and energy dissipation changes. Bacteria-spheroid interactions resulted in decreased resonance frequency and an increase in dissipation energy for both MRSA and MSSA strains. Following the bacterial binding, these sensors were further exposed to a flow of the penicillin-binding protein (PBP 2a) specific antibody conjugated latex beads. Sensors tested with MRSA responded to PBP 2a antibody beads; while sensors examined with MSSA gave no response. This experimental difference establishes an unambiguous discrimination between methicillin resistant and sensitive...Continue Reading

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Citations

Jun 15, 2014·Journal of Microbiological Methods·Célia SousaHubert Perrot
Jul 7, 2014·Colloids and Surfaces. B, Biointerfaces·Zeinab HosseinidoustNathalie Tufenkji
Sep 4, 2015·Virology·Zachary J Storms, Dominic Sauvageau
Oct 17, 2015·Analytical and Bioanalytical Chemistry·Riikka PeltomaaMaría Cruz Moreno-Bondi
Mar 13, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Daniel V DixonNathalie Tufenkji
Feb 11, 2014·Expert Review of Medical Devices·Irina SorokulovaVitaly Vodyanoy

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