Detection and identification of mycobacteria by amplification of mycobacterial DNA

Molecular Microbiology
A J HanceB Gicquel

Abstract

A 383bp segment of the gene coding for the 65kD mycobacterial antigens from Mycobacterium tuberculosis, Mycobacterium bovis, Mycobacterium avium, Mycobacterium paratuberculosis, and Mycobacterium fortuitum was amplified using Taq polymerase and synthetic oligonucleotide primers and the amplified DNAs from four of these species were compared by nucleotide sequencing. Although the gene segments from these species showed considerable similarity, oligonucleotide probes which could distinguish M. tuberculosis/M. bovis, M. avium/M. paratuberculosis and M. fortuitum could be identified. Samples containing 10(6) human cells and serial dilutions of a suspension of intact mycobacteria were prepared, DNA was extracted, the segment of the mycobacterial DNA sequence amplified, and the amplified DNA hybridized with oligonucleotide probes. In two independent experiments, this procedure permitted the detection and identification of less than 100 mycobacteria in the original sample. These results suggest that this approach may prove useful in the early diagnosis of mycobacterial infection.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·V MehraR A Young
Feb 1, 1986·Journal of General Microbiology·R PatelP Mounts
Mar 1, 1987·Journal of Bacteriology·T M Shinnick
Aug 1, 1987·Journal of Clinical Microbiology·T A DrakeD A Bruckner
Jan 1, 1988·Journal of Biological Standardization·M GheorghiuC Fillastre
Aug 1, 1986·Annals of Internal Medicine·C C HawkinsD Armstrong
Dec 1, 1985·Infection and Immunity·J E TholeJ D van Embden
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·D DiMaioT Maniatis
Jun 26, 1980·The New England Journal of Medicine·J GlassrothD E Snider

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Citations

Aug 26, 2003·Journal of Clinical Laboratory Analysis·Sanjay K GargPrakash S Bisen
Jun 1, 1996·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·J J PalaciosJ F de Quirós
Jul 1, 1996·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M A De FrancescoA Turano
Mar 1, 1992·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·A PeneauJ P Moisan
Oct 1, 1994·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·S EhlersH Hahn
Sep 1, 1990·Indian Journal of Pediatrics·A Rattan
Aug 1, 2000·Indian Journal of Clinical Biochemistry : IJCB·M Sritharan, V Sritharan
Jul 1, 2000·Indian Journal of Clinical Biochemistry : IJCB·S KamatchiammalV Sritharan
Jun 20, 1992·Lancet·J GerdesH Magnussen
Jan 23, 1993·Lancet·K DegitzM Volkenandt
Jan 1, 1994·Diagnostic Microbiology and Infectious Disease·O LunguS Silverstein
Jul 1, 1991·Pediatric Neurology·R G Curless, C D Mitchell
Mar 1, 1994·Research in Microbiology·J T Crawford
Mar 1, 1995·Research in Microbiology·M Picardeau, V Vincent
Feb 1, 1991·Current Opinion in Biotechnology·J C Richards
Dec 27, 2005·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Eric CrubézyBertrand Ludes
Feb 18, 2004·Journal of Microbiological Methods·John IkonomopoulosVassilis Gorgoulis
Mar 1, 2002·Journal of Microbiological Methods·Vanda Dolabela de MagalhãesMarinês Dalla Valle Martino
Apr 15, 2000·Diagnostic Microbiology and Infectious Disease·A Waléria-AleixoP C Ferreira
Jan 8, 1999·Comptes rendus de l'Académie des sciences. Série III, Sciences de la vie·E CrubézyD Montagnon
Jan 29, 2000·The British Journal of Dermatology·S K AroraS Sehgal
Dec 12, 2000·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·D Gomez-PastranaJ Navarro
Aug 1, 1992·Letters in Applied Microbiology·G RodrigoS B Svenson
May 1, 1997·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·H Soini, M K Viljanen
Aug 1, 1997·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·J C RodríguezG Royo
Dec 2, 1998·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·C E SonckT O Ekfors
Dec 24, 1997·Australian Veterinary Journal·E ArielB L Roberts
Jun 28, 2001·Journal of Clinical Microbiology·P S Conville, F G Witebsky
Jun 7, 2003·Journal of Clinical Microbiology·Masao FukushimaRyuji Kawaguchi

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