PMID: 9542930May 23, 1998Paper

Detection and identification of Mycobacterium tuberculosis directly from sputum sediments by ligase chain reaction

Journal of Clinical Microbiology
D F Moore, J I Curry

Abstract

Sputum specimens received for the diagnosis of tuberculosis or other mycobacterial infections were tested by a ligase chain reaction (LCR)-based assay and acid-fast stain and culture techniques. Results from the LCR assay (Abbott LCx Mycobacterium tuberculosis [MTB] Assay) were compared to results from standard culture techniques held for 6 weeks. Four hundred ninety-three specimens from 205 patients suspected of pulmonary tuberculosis were included in the prospective study. Thirty-four (6.9%) of the specimens were culture positive for M. tuberculosis, and 13 (38%) of these were also fluorochrome stain positive. LCR sensitivities and specificities compared to culture were 74 and 98%, respectively. LCR sensitivity was 100% for fluorochrome stain-positive specimens and 57% for fluorochrome stain-negative specimens. Nine LCR-negative, culture-positive specimens were the result of low concentrations of M. tuberculosis. No inhibitors were detected in any of these specimens. Of the eight LCR-positive, culture-negative specimens, five were from patients with active tuberculosis. With these considered culture misses, final LCR sensitivity, specificity, positive predictive value, and negative predictive value were 77, 99, 91, and 98%, r...Continue Reading

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Citations

May 6, 1999·Current Opinion in Pulmonary Medicine·S Gillespie
Apr 14, 2016·Indian Journal of Clinical Biochemistry : IJCB·Narotam SharmaPraveen Sharma
May 10, 2008·American Journal of Respiratory and Critical Care Medicine·Michael CamposDavid Ashkin
Dec 10, 2003·Journal of Clinical Microbiology·Claudio Piersimoni, Claudio Scarparo
Mar 30, 2002·Journal of Clinical Microbiology·Patrick C Y WooKwok-Yung Yuen
Aug 31, 2002·Journal of Clinical Microbiology·Jacqueline LachnikFranz-Christoph Bange
May 17, 2005·Diagnostic Microbiology and Infectious Disease·Douglas F MooreLydia T Mikhail
Aug 18, 2021·Clinical Case Reports·Rajan ChamlagainBasanta Sharma Paudel
Jul 5, 2008·Journal of Microbiological Methods·Ioannis K NeonakisDemetrios A Spandidos

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