Detection and identification of the Candida species by 25S ribosomal DNA analysis in the urine of candidal cystitis

The Journal of Veterinary Medical Science
Rui KanoAtsuhiko Hasegawa

Abstract

Candida species in clinical urine samples were identified directly by the newly developed method of PCR analysis on 25S ribosomal DNA (rDNA). Two dogs were referred to the Animal Medical Center, Nihon University School of Veterinary Medicine, Fujisawa, Kanagawa, Japan for the examination of chronic cystitis. Microscopic examination of urine samples from these dogs revealed yeast cells. Urine culture on Sabouraud's dextrose agar at 27 degrees C for 5 days produced white to cream colored colonies. The isolates were identifical to Candida albicans and C. parapsilosis by mycological examination, respectively. The nucleotide sequences of 25S ribosomal DNA from these urine isolates showed 99% similarity to those of a reference strain of Candida albicans or C. parapsilosis. The nucleotide sequences of 25S rDNA obtained directly from urine samples were also identical to C. albicans and C. parapsilosis, respectively. Confirming the results on the isolates cultured from the same urine samples. This PCR analysis method could be available for the direct identification of Candida species in urine samples within 2 days.

References

Jul 1, 1992·Age and Ageing·P A O'NeillD Bennett
May 1, 1994·Journal of Medical Microbiology·K MakimuraH Yamaguchi
Mar 13, 1999·Journal of Clinical Microbiology·M BougnouxM Nicolas-Chanoine
May 13, 1999·International Journal of Systematic Bacteriology·J W FellT Boekhout

❮ Previous
Next ❯

Citations

May 12, 2009·Nihon Ishinkin Gakkai zasshi = Japanese journal of medical mycology·Ken OkabayashiToshi Watanabe
Jan 10, 2009·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·N KondoriI Mattsby-Baltzer
Mar 15, 2018·Medical Mycology·Seyedmojtaba SeyedmousaviJacques Guillot
Jul 12, 2002·Yeast
Sep 20, 2005·Mycopathologia·Hiroko OzawaAtsuhiko Hasegawa
Oct 31, 2017·Tropical Animal Health and Production·Ugur ParinGamze Balat

❮ Previous
Next ❯

Related Concepts

Related Feeds

Candidiasis

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Candidiasis (ASM)

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Candida albicans

Candida albicans is an opportunistic, fungal pathogen of humans that frequently causes superficial infections of oral and vaginal mucosal surfaces of debilitated and susceptible individuals. Discover the latest research on Candida albicans here.

Related Papers

Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
Claudia CafarchiaDomenico Otranto
© 2022 Meta ULC. All rights reserved