Detection and identification of viruses by electron microscopy

Journal of Electron Microscopy Technique
Sara E Miller

Abstract

Electron microscopy can aid in the rapid diagnosis of viral diseases, as it can be performed in a matter of hours, but on a routine basis it should be used in conjunction with other techniques. Initially, the specimen source and patient symptoms should be ascertained, as these will lend suggestions of possible agents while eliminating others; however, this information should not be allowed to prejudice observation in such a way as to cause oversight of an unlikely pathogen. Second, selection of the method of preparation should be based on sample consistency; extraction, debris clarification, concentration, tissue culture amplification, or embedment may be necessary. Finally, false-positive results must be avoided by differentiating viruses from cell organelles or debris, mycoplasmal or bacterial contamination, and bacteriophages.

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Citations

Nov 9, 2000·The New England Journal of Medicine·K M BeckerJ N MacCormack
Jan 1, 1988·Journal of Electron Microscopy Technique·S E Miller, D N Howell
Jan 1, 1997·Annals of Saudi Medicine·S E Miller
Oct 14, 2009·Clinical Microbiology Reviews·Cynthia S Goldsmith, Sara E Miller
Oct 10, 2001·Journal of American College Health : J of ACH·C L MoeS E Miller
Dec 9, 2016·Journal of Virology·Oluwapelumi O AdeyemiDavid J Rowlands
Jul 12, 2020·Journal of the American Society of Nephrology : JASN·Cynthia S Goldsmith, Sara E Miller
Oct 21, 2020·Viruses·Elad MilrotOrly Laskar

Related Concepts

Bacteriophages
Diagnosis
Electron Microscopy
Influenza A virus
Tissue Culture
Virus Diseases
Pleuropneumonia-Like Organisms
Extraction
False Positive
Source

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