Detection and interpretation of lysergic acid diethylamide results by immunoassay screening of urine in various testing groups

Journal of Analytical Toxicology
A H WuJ Brown

Abstract

A total of 2259 urine samples were assayed for lysergic acid diethylamide (LSD) using radioimmunoassay (RIA, Coat-a-Count, Diagnostics Products) and a premarket cloned enzyme donor immunoassay (CEDIA, Boehringer Mannheim). Urine samples were obtained from patients admitted to the emergency room, patients in drug rehabilitation programs, and adults and juveniles in criminal probation programs. An overall incidence of positive results was 0.80% for CEDIA (500-pg/mL cutoff) and 0.89% and 0.18% for RIA at cutoffs of 250 and 500 pg/mL, respectively. Of the CEDIA-positive samples, only 17 and 11% were positive by RIA at 250 and 500 pg/mL, respectively, whereas among RIA-positive samples, only 10% of those > 250 pg/mL and only 25% of those > 500 pg/mL were positive by CEDIA. Moreover, only 2 of 25 of samples positive by one of these screening assays were confirmed by gas chromatography-mass spectrometry (GC-MS). It is likely that discrepancies in results between immunoassays are due to differences in antibody specificities used to detect LSD metabolites. In addition, immunoassays may be more sensitive than GC-MS for detecting LSD use as current confirmation assays are targeted towards detection of the parent drug only. The interpretat...Continue Reading

Citations

Aug 13, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·S SchneiderR Wennig
Nov 26, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·P Marquet, G Lachâtre
Nov 26, 1999·Journal of Chromatography. B, Biomedical Sciences and Applications·S A ReuschelR L Foltz
Jun 29, 1999·Analytical Chemistry·T A BrettellR Saferstein

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