Detection and localization of protein modifications by high resolution tandem mass spectrometry

Mass Spectrometry Reviews
Fanyu MengNeil L Kelleher

Abstract

For interrogation of peptides with diverse modifications, no other instrument is as versatile as the Fourier-transform mass spectrometer (FTMS). Particularly using electrospray ionization (ESI), many intact proteins and their proteolytic products harboring post-translational and chemical modifications (PTMs) have been studied by high resolution tandem mass spectrometry (MS/MS). The widely touted analytical figures of merit for FTMS in fact have translated into clarity when analyzing PTMs from phosphorylations to disulfides, oxidations, methylations, acetylations, and even exotic PTMs found in the biosynthesis of antibiotics and other natural products. A top down approach to PTM detection and localization is proving extensible to an increasing variety of PTMs, some of which are stable to MS/MS at the protein level but unstable to amide bond cleavage by threshold dissociations at the level of small peptides <3 kDa. In contrast, MS/MS using electron capture dissociation (ECD) allows precise localization of even labile PTMs given enough sample and abundant molecular ions. Finally, this brief synopsis of recent literature highlights specific PTMs that perturb the protein backbone therefore altering MS/MS fragmentation patterns. Thus...Continue Reading

References

Jan 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J A LooF W McLafferty
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·K D HenryD F Hunt
Aug 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·E MørtzM Mann
Oct 10, 1997·Analytical Chemistry·J QinB T Chait
Oct 13, 1998·Mass Spectrometry Reviews·A G MarshallG S Jackson
Sep 28, 2000·Rapid Communications in Mass Spectrometry : RCM·A StensballeR A Zubarev
Feb 14, 2002·Proceedings of the National Academy of Sciences of the United States of America·Siu Kwan SzeFred W McLafferty
Mar 5, 2002·Nature Biotechnology·Scott B FicarroForest M White
Jun 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Michael J MacCossJohn R Yates
Jul 19, 2002·Journal of Mass Spectrometry : JMS·Gavin E Reid, Scott A McLuckey
Dec 18, 2002·Journal of the American Society for Mass Spectrometry·Ziqiang Guan
Mar 1, 2003·Nature Biotechnology·Matthias Mann, Ole N Jensen
Mar 22, 2003·Journal of the American Society for Mass Spectrometry·Ying GeClifton E Barry
Nov 25, 2003·Analytical Chemistry·Gregory K TaylorNeil L Kelleher
Oct 1, 1995·Journal of the American Society for Mass Spectrometry·N L KelleherF W McLafferty

❮ Previous
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Citations

Sep 6, 2007·Analytical and Bioanalytical Chemistry·E D RaczyńskaM Szelag
Aug 19, 2007·World Journal of Urology·Dan Theodorescu, Harald Mischak
Jun 24, 2006·Biotechnology Letters·Ray Bakhtiar, Ziqiang Guan
Apr 8, 2011·Journal of the American Society for Mass Spectrometry·Séverine ZirahJean-Claude Tabet
Apr 8, 2011·Journal of the American Society for Mass Spectrometry·Jenna ScotcherPat R R Langridge-Smith
Sep 29, 2011·Journal of the American Society for Mass Spectrometry·David J ClarkePat R R Langridge-Smith
Jun 7, 2012·Journal of the American Society for Mass Spectrometry·Jiawei ChenMichael L Gross
Sep 6, 2012·Journal of the American Society for Mass Spectrometry·Brittany L Heath, Rebecca A Jockusch
Sep 6, 2006·Journal of the American Society for Mass Spectrometry·Haichuan Liu, Kristina Håkansson
Jul 14, 2007·Journal of the American Society for Mass Spectrometry·Qingchun ZhangYinsheng Wang
Apr 11, 2008·Journal of the American Society for Mass Spectrometry·Cheng LinPeter B O'Connor
Jun 24, 2008·Journal of the American Society for Mass Spectrometry·Kathrin BreukerFred W McLafferty
Jul 29, 2008·Journal of the American Society for Mass Spectrometry·Xiaojuan LiPeter B O'Connor
Mar 20, 2009·Journal of the American Society for Mass Spectrometry·Hisham Ben HamidaneYury O Tsybin
Mar 13, 2010·Journal of the American Society for Mass Spectrometry·Jiang ZhangYing Ge
Oct 7, 2008·The Journal of Physical Chemistry. a·Anne I S HolmEvan R Williams
Dec 5, 2008·Journal of Proteome Research·Michael T BoyneNeil L Kelleher
May 6, 2011·Molecular & Cellular Proteomics : MCP·Charity T AikenLan Huang
Dec 26, 2008·Briefings in Functional Genomics & Proteomics·Kevin Blackburn, Michael B Goshe
Oct 31, 2007·Molecular and Cellular Biology·James J PesaventoCraig A Mizzen
Sep 9, 2006·Microbiology and Molecular Biology Reviews : MMBR·Paul O HassaMichael O Hottiger
Jul 19, 2008·Annual Review of Analytical Chemistry·Alan G Marshall, Christopher L Hendrickson
Sep 7, 2007·Proceedings of the National Academy of Sciences of the United States of America·Julia Chamot-RookeGuillaume Duménil
May 30, 2009·Analytical and Bioanalytical Chemistry·Jeffery S EdmistonWillie J McKinney
Feb 14, 2013·FEBS Letters·Adam P LothropStephen M Fuchs
Apr 12, 2007·Expert Review of Proteomics·Steve M M Sweet, Helen J Cooper
Jun 6, 2009·Expert Review of Proteomics·Christopher C ParkDesmond J Smith
Jun 22, 2005·Expert Review of Proteomics·Thomas Kislinger, Andrew Emili
Dec 29, 2015·Chemical Reviews·Sophie DallongevilleCaroline Tokarski
Feb 24, 2009·Protein Expression and Purification·Juan Casado-VelaJ Ignacio Casal
Nov 19, 2008·Mass Spectrometry Reviews·Vanessa Leah Mendoza, Richard W Vachet
Aug 23, 2008·Mass Spectrometry Reviews·Zhongqi ZhangXiaoyu Chen
Apr 6, 2007·Mass Spectrometry Reviews·Catherine A Srebalus Barnes, Amareth Lim
May 23, 2006·Rapid Communications in Mass Spectrometry : RCM·Satoko AkashiYoshifumi Nishimura

❮ Previous
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