Abstract
Umbelopsis ramanniana is an oleaginous fungus belonging to the Mucoromycotina subphylum. Our group had previously detected four double-stranded RNA (dsRNA) bands in the U. ramanniana NRRL 1296 strain by gel electrophoresis. Here we describe the molecular characterization of its dsRNA elements as well as the discovery of four novel dsRNA viruses: Umbelopsis ramanniana virus 1 (UrV1), Umbelopsis ramanniana virus 2 (UrV2), Umbelopsis ramanniana virus 3 (UrV3), and Umbelopsis ramanniana virus 4 (UrV4). Full genomes of UrV1, UrV3, and UrV4 were determined using the full-length amplification of cDNAs (FLAC) technique; they contain two open reading frames (ORF), which putatively encode the coat protein (CP) and the RNA dependent RNA polymerase (RdRp), respectively. In case of UrV2, a partial ORF encoding a partial RdRp gene could be determined. Based on the phylogeny inferred from the RdRp sequences, UrV1 and UrV4 belong to the genus Totivirus, while UrV2 may belong to the genus Victorivirus. UrV3 nested to a novel, unclassified group of Totiviridae, which is related to the genus Totivirus. Hybridization analysis revealed that the dsRNA molecules of UrV1 and UrV4 correspond to the same 5.0-kbp electrophoretic band, whilst the probe fo...Continue Reading
References
Dec 11, 1993·Nucleic Acids Research·J A Bruenn
Mar 17, 1998·Virology·Y HongK W Buck
Apr 29, 1998·Journal of Virology·E Routhier, J A Bruenn
May 1, 1998·Virus Genes·S A Ghabrial
Aug 26, 1998·Antonie van Leeuwenhoek·C VágvölgyiC Fekete
Jun 13, 2001·Canadian Journal of Microbiology·T PappC Vágvölgyi
Jun 27, 2003·Mycological Research·Wieland Meyer, Walter Gams
Jul 5, 2005·Bioinformatics·Chun-Hsiang HuangHsien-Tai Chiu
Aug 3, 2005·Nature Reviews. Microbiology·Donald L Nuss
Apr 17, 2007·Journal of Virological Methods·Sushila MaanPeter Paul Clement Mertens
Apr 30, 2009·Annual Review of Phytopathology·Said A Ghabrial, Nobuhiro Suzuki
Aug 24, 2010·Journal of Virological Methods·Omar DarissaGünter Adam
May 6, 2011·Journal of Virology·Hua LiSaid A Ghabrial
Sep 25, 2012·Applied and Environmental Microbiology·Noemí HerreroIñigo Zabalgogeazcoa
Jan 19, 2013·Molecular Biology and Evolution·Kazutaka Katoh, Daron M Standley
Mar 19, 2013·Advances in Virus Research·Bradley I Hillman, Guohong Cai
Jul 16, 2013·Virus Research·Liqiang LiXiwu Zhu
Jan 24, 2014·Bioinformatics·Alexandros Stamatakis
Mar 1, 2014·Genome Announcements·Itaru TakedaMasayuki Machida
Mar 17, 2015·Virology·Said A GhabrialNobuhiro Suzuki
Nov 6, 2015·PLoS Pathogens·Moonil SonKook-Hyung Kim
Nov 13, 2015·Archives of Virology·Sha ChenXueping Zhou
Jan 19, 2016·Frontiers in Microbiology·Tim LachnitPeter Steinberg
Feb 16, 2016·Microbes and Environments·Syun-Ichi UrayamaTakuro Nunoura
Jun 3, 2016·PLoS Pathogens·Sonia CampoJames C Carrington
Oct 16, 2016·Mycologia·Joseph W SpataforaJason E Stajich
Nov 24, 2016·Nature·Mang ShiYong-Zhen Zhang
Dec 28, 2016·Database : the Journal of Biological Databases and Curation·Matej StanoLubos Klucar
Jan 15, 2017·FEMS Microbiology Ecology·Eeva J VainioJarkko Hantula
Jan 3, 2019·BioRxiv : the Preprint Server for Biology·K. GilbertJames Carrington