Abstract
To reveal the distribution of tick-borne parasites, we established a novel nested polymerase chain reaction (PCR) system to detect the most common agents of tick-borne parasitic diseases, namely Babesia, Theileria, and Hepatozoon parasites. We collected host-seeking or animal-feeding ticks in Kagoshima Prefecture, the southernmost region of Kyusyu Island in southwestern Japan. Twenty of the total of 776 tick samples displayed a specific band of the appropriate size (approximately 1.4-1.6kbp) for the 18S rRNA genes in the novel nested PCR (20/776: 2.58%). These PCR products have individual sequences of Babesia spp. (from 8 ticks), Theileria spp. (from 9 ticks: one tick sample including at least two Theileria spp. sequences), and Hepatozoon spp. (from 3 ticks). Phylogenetic analyses revealed that these sequences were close to those of undescribed Babesia spp. detected in feral raccoons in Japan (5 sequences; 3 sequences being identical), Babesia gibsoni-like parasites detected in pigs in China (3 sequences; all sequences being identical), Theileria spp. detected in sika deer in Japan and China (10 sequences; 2 sequences being identical), Hepatozoon canis (one sequence), and Hepatozoon spp. detected in Japanese martens in Japan (t...Continue Reading
Citations
Sep 25, 2018·Transboundary and Emerging Diseases·Hongfei ShiLunguang Yao
Jan 9, 2019·Parasites & Vectors·Alejandro Cabezas-CruzSara Moutailler
Oct 8, 2018·Parasitology Research·Jie CuiJunlong Zhao
Sep 7, 2018·Parasitology Research·Saw BawmKen Katakura
Oct 4, 2020·Experimental & Applied Acarology·Elzahara ElbazToshio Tsubota
Dec 30, 2020·Ticks and Tick-borne Diseases·Elisha ChatangaRyo Nakao
Feb 5, 2021·Parasitology International·Saw BawmKen Katakura
Nov 24, 2020·Parasitology Research·Elisha ChatangaRyo Nakao
Mar 7, 2021·Pathogens·ThankGod E OnyicheCornelia Silaghi
Mar 8, 2021·Ticks and Tick-borne Diseases·Momoko MorikawaTatsunori Masatani
Apr 7, 2018·Ticks and Tick-borne Diseases·Yongjin QiuRyo Nakao
Apr 4, 2021·Pathogens·Ghania BoulariasSara Moutailler
Apr 19, 2021·Parasitology International·Rie MarukoShin-Ichi Nakamura
May 13, 2021·Acta Tropica·Huynh Luu Cam TuMorakot Kaewthamasorn
May 14, 2021·Ticks and Tick-borne Diseases·Saw BawmKen Katakura
May 22, 2021·Parasites & Vectors·Ahmet Efe KöseoğluCemal Ün
Jun 14, 2021·Veterinary Parasitology·Xiaomeng AnJunlong Zhao
Aug 29, 2021·Pathogens·Ghofrane BaltiYoumna M'ghirbi
Sep 30, 2021·Parasites & Vectors·Anna Bajer, Dorota Dwużnik-Szarek
Nov 3, 2021·Parasitology Research·Yongjin QiuHisao Tamura